%0 Journal Article %A Takeda, Hisao %A Oya, Akira %A Hashimoto, Katsuyuki %A Yamada, Masa-Atsu %T Intracellular Distribution of Virus-Specific RNA in Chick Embryo Cells Infected With Japanese Encephalitis Virus %D 1977 %J Journal of General Virology, %V 34 %N 1 %P 201-205 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-34-1-201 %I Microbiology Society, %X SUMMARY Japanese encephalitis virus (JEV) infected chick embryo (CE) cells were treated with 4 µg actinomycin D/ml and 5 mm-d-glucosamine at 2 or 3 h before harvesting. Production of JEV was not affected by the short-time treatment of these drugs. The radioactivity in virus-specific RNA in the glucosamine-treated cells was apparently higher than in non-treated cells. Nuclear and cytoplasmic extracts were prepared from the JEV-infected cells pulse-labelled with 3H-uridine at 15 h after infection. Analysis of virus RNA in nuclear extracts on sucrose density gradients showed that most of the radioactivity was in 23S RNA, 26S RNA and 8 to 12S RNA. The radioactivity of virus RNA in cytoplasmic extracts was found in 42S RNA and RNA fragments sedimenting at less than 8S. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-34-1-201