1887

Abstract

SUMMARY

A solid-phase radioimmunoassay (RIA) was used to determine the presence of IgG and IgM antibodies to measles virus in human serum and cerebrospinal fluid (CSF). Purified measles virus was adsorbed on to polystyrene balls, which were then exposed to serial dilutions of test serum or CSF. The presence of antibody was measured by its capacity to bind I-labelled specific anti-human IgG or IgM.

Serum from a variety of patients as well as measles-immune clinically healthy persons were tested; binding ratios (using negative human serum controls) were usually between 10 and 30, but with subactue sclerosing panencephalitis (SSPE) ratios were as high as 50. Often CSF specimens tested, all but one, which was taken early in the convalescent phase of measles infection, had detectable IgG antibody. In six patients with acute measles, IgM antibodies were found in all serum specimens taken one or more days after the onset of rash. Maximal titres of 1:10000 to 1:40000 were found about 7 days later. Thereafter, IgM titres decreased rapidly but were still detectable at 40 days. A purified ribonucleoprotein of measles virus was also used successfully as an antigen in this RIA method.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-1-167
1977-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/1/JV034001167.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-1-167&mimeType=html&fmt=ahah

References

  1. Cameron C. H., Dane D. S. 1974; Viruses. In radioimmunoassay and saturation analysis. British Medical Bulletin 30:90–92
    [Google Scholar]
  2. Casey H. L. 1965; Adaptation of the LBCF method to microtechnique. In Standardized Diagnostic Complement Fixation Method and Adaptation to Microtest Public Health Monograph no 74 Public Health Service Publication no 1228 Washington, D.C: U.S. Government Printing Office;
    [Google Scholar]
  3. Hunter W. M., Greenwood F. C. 1962; Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature, London 194:495–496
    [Google Scholar]
  4. Kalimo K. O. K., Meurman O. H., Halonen P. E., Ziola B. R., Viljanen M. K., Granfros K., Toivanen P. 1976; Solid-phase radioimmunoassay of rubella virus IgG and IgM antibodies. Journal of Clinical Microbiology 4:117–123
    [Google Scholar]
  5. Norrby E., Gollmar Y. 1972; Appearance and persistence of antibodies against different virus components after regular measles infections. Infection and Immunity 6:240–247
    [Google Scholar]
  6. Prince A. M., Brotman B., Jass D., Ikram H. 1973; Specificity of the direct solid-phase radioimmunoassay for detection of hepatitis-B antigen. Lancet i:1346–1350
    [Google Scholar]
  7. Salmi A. S., Norrby E., Panelius M. 1972; Identification of different measles virus-specific antibodies in the serum and cerebrospinal fluid from patients with subacute sclerosing panencephalitis and multiple sclerosis. Infection and Immunity 6:248–254
    [Google Scholar]
  8. Salmi A., Panelius M., Vainionpaa R. 1974; Antibodies against different viral antigens in cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases. Acta Neurologica Scattdinavica 50:183–193
    [Google Scholar]
  9. Toivanen A., Viljanen M. K., Savilahti E. 1975; IgM and IgG antimilk antibodies measured by radioimmunoassay in myocardial infarction. Lancet ii:205–207
    [Google Scholar]
  10. Viljanen M. K., Grantors K., Toivanen P. 1975; Radioimmunoassay of class-specific antibodies (RIACA): chicken antibodies to bovine serum albumin. Immunochemistry 12:699–705
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-1-167
Loading
/content/journal/jgv/10.1099/0022-1317-34-1-167
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error