The salivary glands of planthoppers, Laodelphax striatellus, transmitting barley yellow striate mosaic virus (BYSMV), cryptogram */*:*/*:U/U:S,I/Au, were sectioned and examined in the electron microscope. BYSMV was detected in the cytoplasm but not in the nuclei or other organelles of infected cells which did not show structural changes. The BYSMV virions, 300 to 320 nm long and about 40 nm wide, were frequently arranged in parallel aggregates bound by a membrane. Long flexuous tubules of variable length, 18 to 20 nm wide were found in close association with BYSMV. The tubules were typically found in bundles surrounded by a membrane which also contained virus particles in different stages of organization. The virion of BYSMV is believed to consist of two coaxial helices, the inner derived from the tubules and the outer being formed between the inner helix and the outer envelope of the virus. A hypothesis is advanced for the morphogenesis of BYSMV in insect tissue which differs from that occurring in plants.
ChenM. J.,
ShikataE.1972; Electron microscopy and recovery of rice transitory yellowing virus from its leafhopper vector, Nephotettix cincticeps. Virology 47:483–486
DalesS.,
ChardonnetY.1973; Early events in the interaction of adenoviruses with HeLa cells. IV. Association with microtubules and the nuclear pore complex during vectorial movement of the inoculum. Virology 56:465–483
GerolaF. M.,
BassiM.1966; An electron microscopy study of leaf vein tumors from maize plants experimentally infected with maize rough dwarf virus. Caryologia 19:13–40
GourantonJ.1972; Development of an intranuclear nonoccluded rod-shaped virus in some midgut cells of an adult insect, Gyrinus natator L. (Coleoplera). Journal of Ultrastructure Research 39:281–294
HugerA. M.1966; A virus disease of the Indian rhinoceros beetle, Oryctes rhinoceros (L.) caused by a new type of insect virus, Rhabdion Virus Oryctes gen. n. sp. n. Journal of Invertebrate Pathology 8:38–51
JonesA. T.,
RobertsI. M.,
MurantA. F.1974; Association of different kinds of bacilliform particle with vein chlorosis and mosaic diseases of raspberry (Rubus idaeus). Annals of Applied Biology 77:283–288
KitajimaE. W.,
LauritisJ. A.,
SwiftH.1969a; Morphology and intracellular localization of a bacilliform latent virus in sweet clover. Journal of Ultrastructure Research 29:141–150
LeclantF.,
SignoretP. A.1976; Premières resultats concernant la transmission d’un rhabdovirus du blé par le delphacide Laodelphax striatellus Fall. Compte Rendu IVe Journées Phytiatrie et Phytopharmacie Circum Mediterraneenes Montpellier, Sept. 1974 (in the press)
MurphyF. A.,
JohnsonH. N.,
HarrisonA. K.,
ShopeR. E.1972; Ultrastructural characterisation of a new member of the Rhabdovirus group-Klamath virus. Archiv für die gesamte Virusforschung 37:323–331
ShopeR. E.,
MurphyF. A.,
HarrisonA. K.,
CauseyO. R.,
KempG. E.,
SimpsonD. T. H.,
MooreD. L.1970; Two African viruses serologically and morphologically related to rabies virus. Journal of Virology 6:690–692
SignoretP. A.,
GiannottiJ.,
AlliotB.1972; Particules de type viral chez Triticum durum Desf. presentant des symptômes de stries chlorotiques. Annales de Phytopathologie 4:45–53
SogawaK.1965; Studies on the salivary glands of rice plant leafhoppers. I. Morphology and histology. Japanese Journal of Applied Entomology and Zoology 9:275–290
TinsleyT. W.,
HarrapK. A.1972; Viruses in vectors and other hosts. In Moving Frontiers in Invertebrate Virology Monographs in Virology, vol 6: pp 27–59 Edited by
MelnickJ. L.
VelaA.,
LeeP. E.1974; Morphogenesis of wheat striate mosaic virus - the internal nucleoprotein component. Journal of Ultrastructure Research 47:169–178