1887

Abstract

SUMMARY

Human fibroblast and mouse L929 cell interferons can be purified by adsorption to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 × 10 units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-3-517
1976-12-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/33/3/JV0330030517.html?itemId=/content/journal/jgv/10.1099/0022-1317-33-3-517&mimeType=html&fmt=ahah

References

  1. Anfinsen C. B., Bose S., Corley L., Gurari-Rotman D. 1974; Partial purification of human interferon by affinity chromatography. Proceedings of the National Academy of Sciences of the United States of America 71:3139–3142
    [Google Scholar]
  2. Berg K., Ogburn C. A., Paucker K., Mogensen K. E., Cantell K. 1975; Affinity chromatography of human leukocyte and diploid cell interferons on Sepharose-bound antibodies. Journal of Immunology 114:640–644
    [Google Scholar]
  3. Billiau A., Joniau M., De Somer P. 4973; Mass production of human interferon in diploid cells stimulated by poly-I: C. Journal of General Virology 19:1–8
    [Google Scholar]
  4. Bock H. G., Skene P., Fleischer S., Casstdy P., Harshman S. 1976; Protein purification: adsorption chromatography on controlled pore glass with the use of chaotropic buffers. Science 191:380–383
    [Google Scholar]
  5. Böhlen P., Stein S., Dairman W., Udenfriend S. 1973; Fluorometric assay of proteins in the nanogram range. Archives of Biochemistry and Biophysics 155:213–220
    [Google Scholar]
  6. Cantell K., Hirvonen S., Mogensen K. E., Pyhälä L. 1974; Human leukocyte interferon: production, purification, stability and animal experiments. In The Production and Use of Interferon for the Treatment and Prevention of Human Virus Infections pp 33–38 Edited by Waymouth C. Rockville: The Tissue Culture Association;
    [Google Scholar]
  7. Davey M. W., Huang J. W., Sulkowski E., Carter W. A. 1974; Hydrophobic interaction of human interferon with concanavalin A-agarose. Journal of Biological Chemistry 249:6354–6355
    [Google Scholar]
  8. Davey M. W., Sulkowski E., Carter W. A. 1976a; Purification and characterisation of mouse interferon with novel affinity sorbents. Journal of Virology 17:439–445
    [Google Scholar]
  9. Davey M. W., Sulkowski E., Carter W. A. 1976b; Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction. Biochemistry 15:704–713
    [Google Scholar]
  10. Davies A. 1965; Some properties of calf interferon. Biochemical Journal 95:20P–21P
    [Google Scholar]
  11. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167–199
    [Google Scholar]
  12. Edy V. G., Billiau A., Joniau M., De Somer P. 1974; Stabilisation of mouse and human interferon by acid pH against inactivation due to shaking and guanidine hydrochloride. Proceedings of the Society for Experimental Biology and Medicine 146:249–253
    [Google Scholar]
  13. Edy V. G., Billiau A., De Somer P. 1976; Human fibroblast and leukocyte interferons show different dose-response curves in assay of cell protection. Journal of General Virology 31:251–255
    [Google Scholar]
  14. Fantes K. H. 1973; Purification and physicochemical properties of interferons. In Interferon and Interferon Inducers pp 171–200 Edited by Finter N. B. Amsterdam: North-Holland Publishing Company;
    [Google Scholar]
  15. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  16. Havell E. A., Vilcek J., Falcoff E., Berman B. 1975; Suppression of human interferon production by inhibitors of glycosylation. Virology 63:475–483
    [Google Scholar]
  17. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilcek J. 1975; Two antigenically distinct species of human interferon. Proceedings of the National Academy of Sciences of the United States of America 72:2185–2187
    [Google Scholar]
  18. Jankowski W. J., Davey M. W., O’malley J. A., Sulkowski E., Carter W. A. 1975; Molecular structure of human fibroblast and leukocyte interferons: probe by lectin and hydrophobic chromatography. Journal of Virology 16:1124–1130
    [Google Scholar]
  19. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  20. Knight E. Jun 1976; Interferon: purification and initial characterisation from human diploid cells. Proceedings of the National Academy of Sciences of the United States of America 73:520–523
    [Google Scholar]
  21. Lampson G. P., Tytell A. A., Nemes M. M., Hilleman M. R. 1963; Purification and characterisation of chick embryo interferon. Proceedings of the Society for Experimental Biology and Medicine 112:468–478
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Ogburn C. A., Berg K., Paucker K. 1973; Purification of mouse interferon by affinity chromatography on anti-interferon globulin-Sepharose. Journal of Immunology m:1206–1218
    [Google Scholar]
  24. Sipe J. D., De Maeyer-Guignard J., Fauconnier B., De Maeyer E. 1973; Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent. Proceedings of the National Academy of Sciences of the United States of America 70:1037–1040
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-33-3-517
Loading
/content/journal/jgv/10.1099/0022-1317-33-3-517
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error