Skip to content
1887

Abstract

SUMMARY

Human fibroblast and mouse L929 cell interferons can be purified by adsorption to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 × 10 units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-3-517
1976-12-01
2025-11-14

Metrics

Loading full text...

Full text loading...

References

  1. Anfinsen C. B., Bose S., Corley L., Gurari-Rotman D. 1974; Partial purification of human interferon by affinity chromatography. Proceedings of the National Academy of Sciences of the United States of America 71:3139–3142
    [Google Scholar]
  2. Berg K., Ogburn C. A., Paucker K., Mogensen K. E., Cantell K. 1975; Affinity chromatography of human leukocyte and diploid cell interferons on Sepharose-bound antibodies. Journal of Immunology 114:640–644
    [Google Scholar]
  3. Billiau A., Joniau M., De Somer P. 4973; Mass production of human interferon in diploid cells stimulated by poly-I: C. Journal of General Virology 19:1–8
    [Google Scholar]
  4. Bock H. G., Skene P., Fleischer S., Casstdy P., Harshman S. 1976; Protein purification: adsorption chromatography on controlled pore glass with the use of chaotropic buffers. Science 191:380–383
    [Google Scholar]
  5. Böhlen P., Stein S., Dairman W., Udenfriend S. 1973; Fluorometric assay of proteins in the nanogram range. Archives of Biochemistry and Biophysics 155:213–220
    [Google Scholar]
  6. Cantell K., Hirvonen S., Mogensen K. E., Pyhälä L. 1974; Human leukocyte interferon: production, purification, stability and animal experiments. In The Production and Use of Interferon for the Treatment and Prevention of Human Virus Infections pp 33–38 Edited by Waymouth C. Rockville: The Tissue Culture Association;
    [Google Scholar]
  7. Davey M. W., Huang J. W., Sulkowski E., Carter W. A. 1974; Hydrophobic interaction of human interferon with concanavalin A-agarose. Journal of Biological Chemistry 249:6354–6355
    [Google Scholar]
  8. Davey M. W., Sulkowski E., Carter W. A. 1976a; Purification and characterisation of mouse interferon with novel affinity sorbents. Journal of Virology 17:439–445
    [Google Scholar]
  9. Davey M. W., Sulkowski E., Carter W. A. 1976b; Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction. Biochemistry 15:704–713
    [Google Scholar]
  10. Davies A. 1965; Some properties of calf interferon. Biochemical Journal 95:20P–21P
    [Google Scholar]
  11. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. Journal of Experimental Medicine 99:167–199
    [Google Scholar]
  12. Edy V. G., Billiau A., Joniau M., De Somer P. 1974; Stabilisation of mouse and human interferon by acid pH against inactivation due to shaking and guanidine hydrochloride. Proceedings of the Society for Experimental Biology and Medicine 146:249–253
    [Google Scholar]
  13. Edy V. G., Billiau A., De Somer P. 1976; Human fibroblast and leukocyte interferons show different dose-response curves in assay of cell protection. Journal of General Virology 31:251–255
    [Google Scholar]
  14. Fantes K. H. 1973; Purification and physicochemical properties of interferons. In Interferon and Interferon Inducers pp 171–200 Edited by Finter N. B. Amsterdam: North-Holland Publishing Company;
    [Google Scholar]
  15. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  16. Havell E. A., Vilcek J., Falcoff E., Berman B. 1975; Suppression of human interferon production by inhibitors of glycosylation. Virology 63:475–483
    [Google Scholar]
  17. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilcek J. 1975; Two antigenically distinct species of human interferon. Proceedings of the National Academy of Sciences of the United States of America 72:2185–2187
    [Google Scholar]
  18. Jankowski W. J., Davey M. W., O’malley J. A., Sulkowski E., Carter W. A. 1975; Molecular structure of human fibroblast and leukocyte interferons: probe by lectin and hydrophobic chromatography. Journal of Virology 16:1124–1130
    [Google Scholar]
  19. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  20. Knight E. Jun 1976; Interferon: purification and initial characterisation from human diploid cells. Proceedings of the National Academy of Sciences of the United States of America 73:520–523
    [Google Scholar]
  21. Lampson G. P., Tytell A. A., Nemes M. M., Hilleman M. R. 1963; Purification and characterisation of chick embryo interferon. Proceedings of the Society for Experimental Biology and Medicine 112:468–478
    [Google Scholar]
  22. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  23. Ogburn C. A., Berg K., Paucker K. 1973; Purification of mouse interferon by affinity chromatography on anti-interferon globulin-Sepharose. Journal of Immunology m:1206–1218
    [Google Scholar]
  24. Sipe J. D., De Maeyer-Guignard J., Fauconnier B., De Maeyer E. 1973; Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent. Proceedings of the National Academy of Sciences of the United States of America 70:1037–1040
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-33-3-517
Loading
/content/journal/jgv/10.1099/0022-1317-33-3-517
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An error occurred
Approval was partially successful, following selected items could not be processed due to error