1887

Abstract

SUMMARY

In the present study the extent of endogenous, type-C virus genome transcription in normal and regenerating mouse liver was analysed by using the technique of nucleic acid hybridization. The RNA preparations from regenerating liver tissues collected at various intervals following partial hepatectomy, and from normal liver samples of BALB/c mice, were hybridized to H-DNA complementary to 60 to 70S RNA of an endogenous, N-tropic virus, released spontaneously from BALB/c mouse cells in culture. Although partial transcription of the endogenous virus genome can be clearly detected in normal liver, a significant increase in the level of virus-specific RNA synthesis in the regenerating liver, in comparison to normal liver, is apparent, following partial hepatectomy. This increase in virus-specific RNA synthesis attains its highest level just before the level of DNA synthesis in the regenerating liver reaches its maximum. These observations may indicate a qualitative or quantitative change in the endogenous type-C virus genome transcription pattern in hepatocytes, in response to partial hepatectomy and suggest that this change in the transcription pattern and the initiation of cell proliferation, in regenerating livers, are probably sequential and related events.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-3-411
1976-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/33/3/JV0330030411.html?itemId=/content/journal/jgv/10.1099/0022-1317-33-3-411&mimeType=html&fmt=ahah

References

  1. Abelev G. I. 1971; Alpha-fetoprotein in ontogenesis and its association with malignant tumors. Advances in Cancer Research 14:295–358
    [Google Scholar]
  2. Abelev G. L., Elgort D. A. 1970; Group-specific antigen of murine leukemia viruses in mice of low-leukemic strains. International Journal of Cancer 6:145–152
    [Google Scholar]
  3. Ando T. 1966; A nuclease specific for heat-denatured DNA isolated from a product of Aspergillus oryzae. Biochimica et Biophysica Acta 114:158–168
    [Google Scholar]
  4. Benveniste R. E., Scolnick E. M. 1973; RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA. Virology 51:370–382
    [Google Scholar]
  5. Benveniste R. E., Todaro G. J. 1974; Multiple divergent copies of endogenous C-type virogenes in mammalian cells. Nature, London 252:170–173
    [Google Scholar]
  6. Benveniste R. E., Todaro G. J., Scolnick E. M., Parks W. P. 1973; Partial transcription of murine type-C viral genomes in BALB/c cell lines. Journal of Virology 12:711–720
    [Google Scholar]
  7. Bonney R. J., Hopkins H. S., Walker P. R., Potter V. R. F. 1973; Glycolytic isoenzymes and glycogen metabolism in regenerating liver from rats on controlled feeding schedules. Biochemical Journal 136:115–124
    [Google Scholar]
  8. Bucher N. L. R. 1963; Regeneration of mammalian liver. International Review of Cytology 15:245–300
    [Google Scholar]
  9. Chattopadhyay S. K., Lowy D. R., Teich N. M., Levine A. S., Rowe W. P. 1974; Qualitative and quantitative studies of AKR-type murine leukemia virus sequences in mouse DNA. Cold Spring Harbor Symposium on Quantitative Biology 39:1085–1101
    [Google Scholar]
  10. Church R. B., McCarthy B. J. 1967a; Ribonucleic acid synthesis in regenerating and embryonic liver: IThe synthesis of new species of RNA during regeneration of mouse liver after partial hepatectomy. Journal of Molecular Biology 23:459–475
    [Google Scholar]
  11. Church R. B., McCarthy B. J. 1967b; Ribonucleic acid synthesis in regenerating and embryonic liver: II The synthesis of RNA during embryonic liver development and its relationship to regenerating liver. Journal of Molecular Biology 23:477–486
    [Google Scholar]
  12. Fan H., Baltimore D. 1973; RNA metabolism of murine leukemia virus: detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA. Journal of Molecular Biology 80:93–117
    [Google Scholar]
  13. Fan H., Besmer P. 1975; RNA metabolism of murine leukemia virus. II. Endogenous virus-specific RNA in the uninfected BALB/c cell line JLS-V9. Journal of Virology 15:836–842
    [Google Scholar]
  14. Gelb L. D., Milstien J. B., Martin M. A., Aaronson S. A. 1973; Characterization of murine leukemia virus-specific DNA present in normal mouse cells. Nature New Biology 244:76–79
    [Google Scholar]
  15. Hellstrom I., Hellstrom K. E., Nishioka M. 1975; Reactivity to tumour-associated antigens detected in mice undergoing liver regeneration. Nature, London 253:744–746
    [Google Scholar]
  16. Huebner R. J., Kelloff G. L., SarmA P. S., Lane W. T., Turner H. C. 1970; Group-Specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implications for ontogenesis and oncogenesis. Proceedings of the National Academy of Sciences of the United States of America 67:366–376
    [Google Scholar]
  17. Huebner R. J., Sarma P. S., Kelloff G. J., Gilden R. V., Meier H., Myers D. D., Peters R. L. 1971; Immunological tolerance to RNA tumor virus genome expression: significance of tolerance and partial expressions in embryogenesis and tumorigenesis. Annals of New York Academy of Sciences 181:246–271
    [Google Scholar]
  18. Hwang K. M., Murphree S. A., Shansky C. W., Sartorelli A. C. 1974; Sequential biochemical events related to DNA replication in the regenerating rat liver. Biochimica et Biophysica Acta 366:143–148
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of DNA from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  20. Mukherjee B. B., Mobry P. M. 1975; Variations in hybridization of RNA from different mouse tissues and embryos to endogenous C-type virus DNA transcripts. Journal of General Virology 28:129–135
    [Google Scholar]
  21. Parks W. P., Livingston D. M., Todaro G. J., Benveniste R. E., Scolnick E. M. 1973; Radioimmunoassay of mammalian type-C viral proteins, III. Detection of viral antigen in normal murine cells and tissues. Journal of Experimental Medicine 137:622–635
    [Google Scholar]
  22. Ross J., Tronick S., Scolnick E. M. 1972; Poly-adenylatc-rich RNA in the 70S RNA of murine leukemiasarcoma virus. Virology 49:230–235
    [Google Scholar]
  23. Scolnick E. M., Parks W., KaWakami T., Kohne D., Okabe R., Gilden R., Hatanaka M. 1974; Primate and murine type-C viral nucleic acid association kinetics: analysis of model systems and natural tissues. Journal of Virology 13:363–369
    [Google Scholar]
  24. Shatkin A. J. 1969; Colorimetric reactions of DNA, RNA and protein determinations. In Fundamental Techniques in Virology pp 231–237 Edited by Habel K., Salzman N. London and New York: Academic Press;
    [Google Scholar]
  25. Sherr C. J., Benveniste R. E., Todaro G. J. 1974; Type-C viral expression in primate tissues. Proceedings of the National Academy of Sciences of the United States of America 71:3721–3725
    [Google Scholar]
  26. Stephenson J. R., Aaronson S. A. 1973; Expression of endogenous RNA C-type virus group-specific antigens in mammalian cells. Journal of Virology 12:564–569
    [Google Scholar]
  27. Sutton W. D. 1971; A crude nuclease preparation suitable for use in DNA reassociation experiments. Biochimica et Biophysica Acta 240:522–531
    [Google Scholar]
  28. Todaro G. I. 1972; Spontaneous release of type-C viruses from clonal lines of spontaneously transformed BALB/3T3 cells. Nature, London 240:157–160
    [Google Scholar]
  29. Todaro G. J., Benveniste R. E., Callahan R., Lieber M. M., Sherr C. J. 1974; Endogenous primate and feline type-C viruses. Cold Spring Harbor Symposium on Quantitative Biology 39:1159–1168
    [Google Scholar]
  30. Todaro G. J., Huebner R. J. 1972; The viral oncogene hypothesis: new evidence. Proceedings of the National Academy of Sciences of the United States of America 69:1009–1015
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-33-3-411
Loading
/content/journal/jgv/10.1099/0022-1317-33-3-411
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error