Simultaneous infection with herpes simplex type 1 and type 2 viruses of chick embryo fibroblasts (CEF), which are only permissive for type 2 virus, or rabbit embryo fibroblasts (REF), which are permissive for both virus types, resulted in a marked reduction of type 2 virus production. This effect was dependent on the m.o.i. of type 1, being expressed at a high rather than a low m.o.i. The rate of interference decreased with the prolongation of the interval between infection with type 2 and type 1 viruses. No evidence suggestive of interferon involvement was obtained. Partial inactivation of type 2 virus by ultraviolet irradiation enhanced the inhibitory effect of type 1 virus. On the other hand, u.v. irradiation of type 1 virus resulted in a progressive loss of inhibitory activity. The results of the present experiments suggest that a type 1 genome function is responsible for the interfering effect, and that an early step in the growth of type 2 virus is sensitive to the particular type 1 virus product involved.


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