1887

Abstract

SUMMARY

Mouse L cell interferon induced by Newcastle disease virus was purified by the procedure described previously (Yamamoto . 1974) followed by gel filtration. The two fractions obtained containing interferon species S (36000 daltons) and F (24000 daltons), respectively, were analysed electrophoretically at pH 4.3. or in the presence of sodium dodecyl sulphate (SDS) at pH 7.2. In both fractions, interferon activity was invariably associated with distinct protein bands. In the F-containing fraction there were essentially no other proteins, and in the S-containing fraction, impurity proteins were well separated from the interferon activity. The apparent mol. wt. determined by SDS-gel electrophoresis showed little or no dependence on gel concentration, suggesting that the interferons had low carbohydrate contents, and did not change after reduction with thiol reagents in SDS and urea.

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1976-11-01
2024-11-03
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References

  1. Borečky L., Fuchsberger N., Hájnická V., Stanček D., žemla J. 1972; Distribution of antiviral and cell inhibitory activity in interferon preparations. Acta Virologica 16:356–358
    [Google Scholar]
  2. Carter W. E. 1970; Interferon: evidence for subunit structure. Proceedings of the National Academy of Sciences of the United States of America 67:620–628
    [Google Scholar]
  3. Gresser I., Bandu M. T., Tovey M., Bodo G., Paucker K., Stewart W. E. II 1973; Interferon and cell division. VII. Inhibitory effect of highly purified interferon preparations on the multiplication of leukemia L 1210 cells. Proceedings of the Society for Experimental Biology and Medicine 142:7–10
    [Google Scholar]
  4. Kawade Y. 1973; Purification and characterization of mouse L cell interferon. I. Preliminary studies. Japanese Journal of Microbiology 17:129–140
    [Google Scholar]
  5. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. The Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  6. Matsuzawa T., Kawade Y. 1974; Cell growth inhibitory activity of electrophoretic fractions of L cell interferon preparation. Acta Virologica 18:383–390
    [Google Scholar]
  7. Mogensen K. E., Cantell K. 1974; Human leukocyte interferon: a role for disulphide bonds. The Journal of General Virology 22:95–103
    [Google Scholar]
  8. Ogburn C. A., Berg K., Paucker K. 1973; Purification of mouse interferon by affinity chromatography on anti–interferon globulin–Sepharose. The Journal of Immunology 11:1206–1218
    [Google Scholar]
  9. Ohwaki M., Kawade Y. 1972; Inhibition of multiplication of mouse cells in culture by interferon preparations. Acta Virologica 16:477–486
    [Google Scholar]
  10. Segrest I. P., Jackson R. L. 1972; Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. In Methods in Enzymology vol 27 part B pp 54–63 Edited by Colowick S. P., Kaplan N. O. New York and London: Academic Press;
    [Google Scholar]
  11. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS–polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  12. Stewart W. E. II 1974; Distinct molecular species of interferons. Virology 61:80–86
    [Google Scholar]
  13. Stewart W. E. II, De Clercq E., De Somer P. 1974a; Stabilization of interferons by defensive reversible denaturation. Nature, London 249:460–461
    [Google Scholar]
  14. Stewart W. E. II, De Somer P., De Clercq E. 1974b; Renaturation of inactivated interferons: requirement for reduction of a major component: lack of requirement for reduction of a minor component. The Journal of General Virology 24:567–570
    [Google Scholar]
  15. Suzuki J., Akaboshi T., Kobayashi S. 1974; A rapid and simple method for assaying interferon. Japanese Journal of Microbiology 18:449–456
    [Google Scholar]
  16. Weil R., Dorner F. 1974; Interferon structure: facts and speculation. In Selective Inhibitors of Viral Functions pp 107–121 Edited by Carter W. E. Cleveland: CRC Press;
    [Google Scholar]
  17. Yamamoto Y., Tsukui K., Ohwaki M., Kawade Y. 1974; Electrophoretic characterization of purified mouse L cell interferon of high specific activity. Journal of General Virology 23:23–32
    [Google Scholar]
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