1887

Abstract

SUMMARY

BHK 21 carrier cells persistently infected with VSV Indiana for over 2 years have been shedding generally very low levels of mature infectious virus or mature T particles (averaging less than one-hundredth p.f.u./cell/day) yet most cells are producing virus antigens and are resistant to homologous superinfection. However, large amounts of biologically active T particle RNP can be recovered from cytoplasmic extracts of these carrier cells even at times when they are shedding no detectable infectious virus. This recovered cytoplasmic RNP replicates (with helper B virions) to produce mature T particles, interferes strongly after DEAE dextranfacilitated uptake and, together with B virions, allows the establishment of a persistent carrier state in exposed cells.

No ‘provirus’ DNA copies of the VSV RNA genome are detectable (less than 1/40 copy/cell or 1 copy per 40 cells) in carrier cells after more than 2 years of persistent infection, and all transfection attempts have failed using DNA from these VSV carriers or DNA from carrier cells persistently infected with some other negative strand RNA viruses (measles, mumps, LCM, influenza, rabies).

Infectious viruses shed after more than 1 year from carrier cells originally infected with wild-type B virions are small plaque mutants showing a slight temperature sensitivity. Cured cell populations can be obtained from the long term VSV carrier culture by cloning in the presence or absence of antiviral antibody.

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1976-11-01
2024-12-06
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References

  1. Britten R., Graham D. S., Neufeld B. 1974 In Methods in Enzymology vol 29 pp 363–418 Edited by Grossman L., Moldave K. New York and London: Academic Press;
    [Google Scholar]
  2. Cartwright B., Smale C. J., Brown F. 1970; Dissection of vesicular stomatitis virus into the infective ribonucleoprotein and immunizing components. Journal of General Virology 7:19–32
    [Google Scholar]
  3. Commerford S. L. 1971; Iodination of nucleic acids in vitro. Biochemistry 10:1993–1999
    [Google Scholar]
  4. Crick J., Brown J. 1974; An interfering component of rabies virus which contains RNA. Journal of General Virology 22:147–151
    [Google Scholar]
  5. Doyle M., Holland I. J. 1973; Immunization and prophylaxis in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 70:2105–2108
    [Google Scholar]
  6. Eaton B. T. 1975; Defective interfering particles of Semliki Forest vorus generated in BHK cells do not interfere with viral RNA synthesis in Aedes albopictus cells. Virology 68:534–538
    [Google Scholar]
  7. Fields B. N., Raine C. S. 1974 In Mechanisms of Virus Disease vol 1 chapter IV, pp 161–167 Edited by Fox W. X., Fox C. F. Menlo Park, California: W. A. Benjamin;
    [Google Scholar]
  8. Holland J. J., McLaren L. C. 1959; Improved method for staining cell monolayers for virus plaque counts. Journal of Bacteriology 78:596–597
    [Google Scholar]
  9. Holland J. J., Villarreal L. P. 1974; Persistent noncytocidal vesicular stomatitis virus infections mediated by defective T particles that suppress virion transcriptase. Proceedings of the National Academy of Sciences of the United States of America 71:2956–2960
    [Google Scholar]
  10. Holland J. J., Villarreal L. P. 1975; Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438–449
    [Google Scholar]
  11. Holland J. J., Villarreal L. P., Breindl J. 1976; Factors involved in the generation and replication of rhabdovirus defective T particles. Journal of Virology 17:805–815
    [Google Scholar]
  12. Horta-Barbosa L., Fuccillo D. A., Zeman W., Sever J. L. 1969; Subacute sclerosing panencephalitis: isolation of measles virus from a brain biopsy. Nature, London 221:974
    [Google Scholar]
  13. Huang A. L., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  14. Joseph B., Oldstone M. B. A. 1974; Antibody induced redistribution of measles virus antigens on the cell surface. Journal of Immunology 113:1205–1209
    [Google Scholar]
  15. Joseph B. S., Oldstone M. B. A. 1975; Immunologic injury in measles virus infection. II. Suppression of immune injury through antigenic modulation. Journal of Experimental Medicine 142:864–876
    [Google Scholar]
  16. Joseph B., Perrin L., Oldstone M. B. A. 1976; Measurement of virus antigens on the surface of HeLa cells persistently infected with wild type and vaccine strains of measles virus. Journal of General Virology 30:329–336
    [Google Scholar]
  17. Kawai A., Matsumoto S., Tanabe K. 1975; Characterization of rabies viruses recovered from persistently infected BHK cells. Virology 67:520–533
    [Google Scholar]
  18. Kohne D., Britten R. 1971 In Procedures in Nucleic Acid Research vol 2 pp 500–512 Edited by Cantoni G. Davies D. New York: Harper and Row;
    [Google Scholar]
  19. Lazzarini R. A., Weber G. H., Johnson L. D., Stamminger G. M. 1975; Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle. Journal of Molecular Biology 97:289–307
    [Google Scholar]
  20. Mudd J. A., Leavitt R. W., Kingsbury D. T., Holland J. J. 1973; Natural selection of mutants of vesicular stomatitis virus by cultured cells of Drosophila melanogaster. Journal of General Virology 20:341–351
    [Google Scholar]
  21. Oldstone M. B. A., Dixon F. J. 1971; Acute viral infection: tissue injury mediated by antiviral antibody through a complement effector system. Journal of Immunology 107:1274–1280
    [Google Scholar]
  22. Palma E. L., Huang A. S. 1974; Cyclic production of vesicular stomatitis caused by defective interfering particles. Journal of Infectious Disease 129:402–410
    [Google Scholar]
  23. Payne F. E., Baublis J. V., Itabashi I. 1969; Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New England Journal of Medicine 281:585
    [Google Scholar]
  24. Perrault J. 1976; Crosslinked double-stranded RNA from a defective vesicular stomatitis virus particle. Virology 70:360–371
    [Google Scholar]
  25. Preble O. T., Youngner J. S. 1973; Temperature-sensitive defect of mutants isolated from L cells persistently infected with Newcastle disease virus. Journal of Virology 12:472–480
    [Google Scholar]
  26. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine, and ethylmethane sulfonate. Journal of Virology 5:559–567
    [Google Scholar]
  27. Reichmann M. E., Villarreal L. P., Kohne D., Lesnaw J., Holland J. J. 1974; RNA polymerase activity and poly (A) synthesizing activity in defective T particles of vesicular stomatitis virus. Virology 58:240–249
    [Google Scholar]
  28. Rodriguez J. E., Henle W. 1965; Studies on persistent infections of tissue cultures. V. The initial stages of infection of L(MCN) cells by Newcastle disease virus. Journal of Experimental Medicine 119:895–921
    [Google Scholar]
  29. Rustigian R. 1966; Persistent infection of cells in culture by measles virus. II. Effect of measles antibody on persistently infected HeLa sublines and recovery of a HeLa clonal line persistently infected with incomplete virus. Journal of Bacteriology 93:1805
    [Google Scholar]
  30. Simpson R. W., Iinuma M. 1975; Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus. Proceedings of the National Academy of Sciences of the United States of America 72:3230–3234
    [Google Scholar]
  31. Sokol F., Kuwert E., Wiktor T. J., Hummeler K., Koprowski H. 1968; Purification of rabies virus grown in tissue culture. Journal of Virology 2:836–849
    [Google Scholar]
  32. Staneck L. D., Trowbridge R. S., Welsh R. M., Wright E. A., Pfau C. J. 1972; Arenaviruses: cellular response to long term in vitro infection with Parana and lymphocytic choriomeningitis viruses. Infection and Immunity 6:444–450
    [Google Scholar]
  33. Stanners C. P., Goldberg V. J. 1975; On the mechanism of neurotropism of vesicular stomatitis virus in newborn hamsters. Studies with temperature sensitive mutants. Journal of General Virology 29:281–296
    [Google Scholar]
  34. Villarreal L. P., Holland J. J. 1976; RNA synthesis in BHK 21 cells persistently infected with vesicular stomatitis virus and rabies virus. Journal of General Virology 33:213–224
    [Google Scholar]
  35. Wagner R. R. 1974; Pathogenicity and immunogenicity for mice of temperature-sensitive mutants of vesicular stomatitis virus. Infection and Immunity 10:309–315
    [Google Scholar]
  36. Wagner R. R., Levy A., Snyder R., Ratcliff G., Hyatt D. 1963; Biologic properties of tWO plaque variants of vesicular stomatitis virus (Indiana Serotype). Journal of Immunology 91:112–122
    [Google Scholar]
  37. Walker D. L. 1964; The viral carrier state in animal cell cultures. Progress in Medical Virology 6:111–148
    [Google Scholar]
  38. Welsh R. M., O’connell C. M., Pfau C. J. 1972; Properties of defective lymphocytic choriomeningitis virus. Journal of General Virology 17:355–359
    [Google Scholar]
  39. Wiktor T. J., Clark H. F. 1972; Chronic rabies virus infection of cell cultures. Infection and Immunity 6:988–995
    [Google Scholar]
  40. Youngner J. S., Quagliana D. O. 1976; Temperature-sensitive mutants of vesicular stomatitis virus and conditionally defective particles that interfere with and are rescued by wild-type virus. Journal of Virology 19:102–107
    [Google Scholar]
  41. Zhdanov V. M. 1975; Integration of viral genomes. Nature, London 256:471–473
    [Google Scholar]
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