RT Journal Article SR Electronic(1) A1 Tanaka, Junji A1 Morita, Osayuki A1 Hatano, MotoichiYR 1976 T1 Factors Involved in the Expression of Cowpox Virus-Specific Antigen in Sendai Virus Carrier Cells JF Journal of General Virology, VO 33 IS 1 SP 87 OP 97 DO https://doi.org/10.1099/0022-1317-33-1-87 PB Microbiology Society, SN 1465-2099, AB SUMMARY Formation of cowpox virus-specific cell surface antigen (CPV S-ag) was enhanced in HVJ (Sendai virus) carrier cells compared to that in parent cells. Temperature shifts from 32 to 35 to 37 °C for these carrier cultures reduced this enhancing activity, making them equivalent to parent cells. The eclipse phase and one-step growth of CPV in the carrier cells were shorter than in normal cells. Extracts of carrier cells exhibited a stimulating activity causing the temporary rise and subsequent lowering of CPV infectivity in their reactions with CPV in vitro, suggesting a cellular acceleration of CPV uncoating. The S-ag forming ability of these carrier cells did not decrease as much as that of parent cells in the presence of actinomycin D or puromycin. The results indicate that persistent infection with HVJ, especially the temperature-sensitive variant, promotes the first step of intracellular growth of CPV, resulting in enhanced formation of S-ag., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-33-1-87