1887

Abstract

SUMMARY

The anticellular and antiviral effects of human leukocyte interferons were studied in the transformed human embryonic cell lines, RSa and RSb. The growth of these cells was inhibited and they began to deteriorate about 48 h after treatment with 500 units/ml of interferon. When interferon was washed out within 48 h, their growth recovered gradually. The effects of interferon on cell growth depended on the amount of interferon added per cell. A subline, named IF, was isolated which grows in the presence of 2000 units/ml of interferon, whereas growth of vesicular stomatitis virus in these cells is suppressed by 10 units/ml of interferon, just as in the parent cells. The anticellular and antiviral effects of interferon on IF cells are discussed in relation to cell surface receptors.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-1-7
1976-10-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/33/1/JV0330010007.html?itemId=/content/journal/jgv/10.1099/0022-1317-33-1-7&mimeType=html&fmt=ahah

References

  1. Adams A., Strander H., Cantell K. 1975; Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. Journal of General Virology 28:207–217
    [Google Scholar]
  2. Berman B., Vilcek J. 1974; Cellular binding characteristics of human interferon. Virology 57:378–386
    [Google Scholar]
  3. Borecky L., Fuchsberoer N., Hajnicka V. 1974; Electrophoretic profiles and activities of human interferon in heterologous cells. Intervirology 3:369–377
    [Google Scholar]
  4. Borecky L., Fuchsberger N., Hajnicka V., Lackovic V. 1973; Antiviral and cell-growth inhibitory effect of interferon. Biomedicine 19:281–286
    [Google Scholar]
  5. Cantell K., Hirvonen S., Mogensen K. E., Pyhälä L. 1974; Human leukocyte interferon: production, purification, stability, and animal experiments. In Interferon, In Vitro monograph No. 3 pp 35–38 Rockville: Tissue Culture Association;
    [Google Scholar]
  6. Chany C., Gregoire A., Vignal M., Lemaitre-Mencuit J., Brown P., Besancon F., Suarez H., Cassingena R. 1973; Mechanism of interferon uptake in parental and somatic monkey-mouse hybrid cells. Proceedings of the National Academy of Sciences of the United States of America 70:55–561
    [Google Scholar]
  7. De Clercq E., Stewart W. E. II 1973; The breadth of interferon action. In Selective Inhibitors of Viral Functions pp 81–106 Edited by Carter W. A. Cleveland: CRC Press;
    [Google Scholar]
  8. Friedman R. M. 1967; Interferon binding: the first step in establishment of antiviral activity. Science, New York 156:1760–1761
    [Google Scholar]
  9. Fuchsberger N., Borecky L., Hajnicka V. 1974; Effect of prolonged interferon treatment on ‘L’ mouse fibroblast cells. Acta Virologica 18:85–87
    [Google Scholar]
  10. Fuchsberger N., Hajnicka V., Borecky L. 1975; Antiviral and cell-growth inhibitory activities of highly purified L-cell interferon. An analysis of quantitative disproportions. Acta Virologica 19:59–66
    [Google Scholar]
  11. Fuse A., Kuwata T. 1976; Effects of interferon on the human clonal line, RSa cells: inhibition of macromolecular synthesis. The Journal of General Virology 32:17–24
    [Google Scholar]
  12. Gresser I. 1972; Antitumor effects of interferon. Advances in Cancer Research 16:97–140
    [Google Scholar]
  13. Gresser I., Bandu M. T., Tovey M., Bodo G., Paucker K., Stewart W. E. II 1973; Interferon and cell division. VII. Inhibitory effect of highly purified interferon preparations on the multiplication of leukemia L1210 cells. Proceedings of the Society for Experimental Biology and Medicine 142:7–10
    [Google Scholar]
  14. Gresser I., Bandu M. T., Brouty-Boyé D. 1974; Interferon and cell division. IX. Interferon-resistant L1210 cells: Characteristics and origin. Journal of the National Cancer Institute 52:553–559
    [Google Scholar]
  15. Gresser I., Brouty-Boyé D., Thomas M.-T., Maceiria-Coelho A. 1970; Interferon and cell division II. Influence of various experimental conditions on the inhibition of L1210 cell multiplication in vitro by interferon preparations. Journal of the National Cancer Institute 45:1145–1153
    [Google Scholar]
  16. Knight E. Jun 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4144
    [Google Scholar]
  17. Kuwata T., Oda T., Sekiya S., Morinaga N. 1976; Characteristics of a human cell line successively transformed by Rous sarcoma virus and simian virus 40. Journal of the National Cancer Institute 56:919–926
    [Google Scholar]
  18. Lee S. H., O’shaughnessy M. V., Rozee K. R. 1972; Interferon induced growth depression in diploid and heteroploid human cells. Proceedings of the Society for Experimental Biology and Medicine 139:1438–1440
    [Google Scholar]
  19. Matsuzawa T., Kawade T. 1974; Cell growth inhibitory activity of electrophoretic reactions of L-cell interferon preparation. Acta Virologica 18:383–390
    [Google Scholar]
  20. Ohwaki M., Kawade Y. 1972; Inhibition of multiplication of mouse cells in culture by interferon preparations. Acta Virologica 16:477–486
    [Google Scholar]
  21. Stewart W. E. II, De Clercq E., De Somer P. 1972; Recovery of cell-bound interferon. Journal of Virology 10:707–712
    [Google Scholar]
  22. Stewart W. E. II 1974; Distinct molecular species of interferons. Virology 61:80–86
    [Google Scholar]
  23. Strander H., Cantell K. 1974; Studies on antiviral and antitumor effects of human leukocyte interferon in vitro and in vivo. In Interferon, In Vitro monograph No. 3 pp 49–56 Rockville: Tissue Culture Association;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-33-1-7
Loading
/content/journal/jgv/10.1099/0022-1317-33-1-7
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error