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Journal of General Virology header logo Journal of General Virology

Volume 33, Issue 1

A Comparison of Human Papovavirus T Antigens No Access

Abstract

SUMMARY

A comparison was made of the T antigens induced in transformed cells or infected permissive cells by representatives of three categories of human papovavirus. The transformed hamster cell lines employed contained T antigen induced by either the BK or RF strains of papovavirus associated with human renal allografts; the JC strain of papovavirus from progressive multifocal leukoencephalopathy (PML), or a variant of SV40 virus isolated from PML. The human papovavirus T antigens were also compared with that of a human cell line transformed by SV40 of simian origin. Anti-T antibody prepared in hamsters against each of the hamster cell lines was absorbed with crude T antigen from each cell line, and the unabsorbed and absorbed antisera were tested for residual T antibody against each cell line, or against infected permissive cells by immunoperoxidase (IP) staining and complement-fixation (CF) tests.

In unabsorbed antisera, T antibodies from each cell line cross-reacted with all T antigens in IP tests, and CF tests showed that T antisera reacted preferentially with T antigen induced by homologous virus.

Absorption studies showed that T antigens from each subdivision had unique antigenic determinants. T antigens of the two urine-derived strains, BK and RF, were identical or nearly so, but were clearly separable from T antigens of JC virus, PML-derived SV40 or simian-derived SV40. JC T antigen was intermediate, being more closely related to T antigens both of BK virus and SV40 virus than the latter were to each other. The T antigen of PML-derived SV40 could be distinguished from the T antigen of simian-derived SV40 and the T antigen of the SV40 variant from human brain was more closely related to those of the other human-derived papovaviruses than was the T antigen of SV40 from monkey kidney.

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  • Accepted:
  • Published Online:
© Journal of General Virology 1976
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1976-10-01
2025-05-12
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References

  1. Adams M. H. 1959 In Bacteriophages New York: Interscience Publishers;
     [Google Scholar]
  2. Coleman D. V., Gardner S. D., Field A. M. 1973; Human polyomavirus infection in renal allograft recipients. British Medical Journal 3:371–375
     [Google Scholar]
  3. Castaigne P., Escourolle R., Derousne C. H., Cathala F., Hauw J.-J., Duclos H., Goust J. M. 1973; Un cas de leucoencephalopathie multifocale progressive. Isolement a partir d’une biopsie cerebrale, d’une nouvelle souche de virus Papova. Revue Neurologique, Paris 128:85–94
     [Google Scholar]
  4. Dougherty R. M. 1976; Induction of tumors in hamsters with a human renal papovavirus, RF strain. Journal of the National Cancer Institute (in the press)
    [Google Scholar]
  5. Dougherty R. M., Dtstefano H. S. 1974; Isolation and characterization of a papovavirus from human urine. Proceedings of the Society for Experimental Biology and Medicine 146:481–487
     [Google Scholar]
  6. Dougherty R. M., Marucci A. A., Distefano H. S. 1972; Application of immunohistochemistry to study of avian leukosis virus. Journal of General Virology 15:149–162
     [Google Scholar]
  7. Field A. M., Gardner S. D., Goodbody R. A., Woodhouse M. A. 1974; Identity of a newly isolated human polyomavirus from a patient with progressive multifocal leucoencephalopathy. Journal of Clinical Pathology 27:341–347
     [Google Scholar]
  8. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (BK) isolated from urine after renal transplantation. Lancet 1:1253–1257
     [Google Scholar]
  9. Girardi A. J., Jensen F. C., Koprowski H. 1965; SV4o-induced transformation of human diploid cells: crisis and recovery. Journal of Cellular and Comparative Physiology 65:69–84
     [Google Scholar]
  10. Graham R. C. Jun, Karnovsky M. J. 1966; The early stages of adsorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. Journal of Histochemistry and Cytochemistry 14:291–302
     [Google Scholar]
  11. Jung M., Krech U., Price P. C., Pyndiah M. N. 1975; Evidence of chronic persistent infections with polyomaviruses (BK type) in renal transplant recipients. Archives of Virology 47:39–46
     [Google Scholar]
  12. Kabat E. A., Mayer M. M. 1961 In Experimental Immmochemistry second edition chapter 4 pp 133–240 Springfield: Illinois: Charles C. Thomas;
     [Google Scholar]
  13. Lecatsas G., Prozesky O. W. 1975; Excretion of morphological variants of human polyoma virus. Archives of Virology 47:393–397
     [Google Scholar]
  14. Major E. O., Di mayorca G. 1973; Malignant transformation of BHK21 clone 13 cells by BK virus - a human papovavirus. Proceedings of the National Academy of Sciences of the United States of America 70:3210–3212
     [Google Scholar]
  15. Mullarkey M. F., Hruska J. F., Takemoto R. K. 1974; Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis. Journal of Virology 13:1014–1019
     [Google Scholar]
  16. Narayan O., Weiner L. P. 1974; Biological properties of two strains of simian virus 40 isolated from patients with progressive multifocal leukoencephalopathy. Infection and Immunity 10:173–179
     [Google Scholar]
  17. Nase L. M., Karkkainen M., Mantyjarvi R. A. 1975; Transplantable hamster tumours induced with the BK virus. Acta Pathologica et Microbioligica Scandinavica 83:347–352
     [Google Scholar]
  18. Osbourn J. E., Robertson S. M., Padgett B. L., Zu Rhein G. M., Walker D. L., Weisblum B. 1974; Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments. Journal of Virology 13:614–622
     [Google Scholar]
  19. Padgett B. L., Walker D. L., Zu Rhein G. M., Eckrokade R. J., Dessel B. H. 1971; Cultivation of papOVa like virus from human brain with progressive multifocal leukoencephalopathy. Lancet 1:1257–1260
     [Google Scholar]
  20. Penney J. B., Narayan O. 1973; Studies of the antigenic relationships of the new human papovaviruses by electron microscopy agglutination. Infection and Immunity 8:299–300
     [Google Scholar]
  21. Portolani M., Barbanti-Brodano G., La Placa M. 1975; Malignant transformation of hamster kidney cells by BK virus. Journal of Virology 15:420–422
     [Google Scholar]
  22. Reese J. M., Reissig M., Daniel R. W., Shah K. V. 1975; Occurrence of BK virus and BK virus-specific antibodies in the urine of patients receiving chemotherapy for malignancy. Infection and Immunity 11:1375–1381
     [Google Scholar]
  23. Sack G. H. Jun, Narayan O., Danna K. J., Weiner L. P., Nathans D. 1973; The nucleic acid of an SV40-like virus isolated from a patient with progressive multifocal leukoencephalopathy. Virology 51:345–350
     [Google Scholar]
  24. Shah K. V., Daniel R. W., Strandberg J. D. 1975; Sarcoma in a hamster inoculated with BK virus, a human papovavirus. Journal of the National Cancer Institute 54:945–950
     [Google Scholar]
  25. Soriano F., Shelburne C. E., Gokcen M. 1974; Simian virus 40 in a human cancer. Nature, London 249:421–424
     [Google Scholar]
  26. Sternberger L. A., Hardy P. H. Jun, Cuculis J. J., Meyer H. G. 1970; The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. Journal of Histochemistry and Cytochemistry 18:315–333
     [Google Scholar]
  27. Takemoto K. K., Martin M. A. 1976; Transformation of hamster kidney cells by BK papovavirus DNA. Journal of Virology 17:247–253
     [Google Scholar]
  28. Takemoto K. K., Mullarkey M. F. 1973; Human papovavirus, BK strain: biological studies including antigenic relationship to simian virus 40. Journal of Virology 12:625–631
     [Google Scholar]
  29. Takemoto K. K., Rabson A. S., Mullarkey M. F., Blaese R. M., Garson C. F., Nelson D. 1974; Isolation of papovavirus from brain tumour and urine of a patient with Wiskott-Aldrich syndrome. Journal of the National Cancer Institute 53:1205–1207
     [Google Scholar]
  30. Walker D. L., Padgett B. L., Zu Rhein G. M., Albert A. E., Marsh R. F. 1973; Human papovavirus JC: induction of brain tumours in hamsters. Science, New York 181:674–676
     [Google Scholar]
  31. Weiner L. P., Herndon R. M., Narayan O., Johnson R. T., Shah K., Rubenstein L. J., Prezeosi T. J., Conley F. K. 1972; Isolation of virus related to SV40 from patients with progressive multifocal leukoencephalopathy. New England Journal of Medicine 286:385–390
     [Google Scholar]
  32. Weiner L. P., Narayan O., Penney J. B., Herndon R. M., Feringa E. R., Tourtellote W. W., Johnson R. T. 1973; Papovavirus of JC type in progressive multifocal leukoencephalopathy. Archives of Neurology 29:1–3
     [Google Scholar]
  33. Weiss A. F., Portmann R., Fischer H., Simon J., Zang K. D. 1975; Simian virus 40-reIated antigens in three human meningiomas with defined chromosome loss. Proceedings of the National Academy of Sciences of the United States of America 72:600–613
     [Google Scholar]
  34. Wright P. J., Bernhardt G., Major E. O., Di Mayorca G. 1976; Comparison ofjthe serology, transforming ability, and polypeptide composition of human papovaviruses isolated from urine. Journal of Virology 17:762–775
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-33-1-61
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A Comparison of Human Papovavirus T Antigens
J. Gen. Virol. 33, 61 (1976); https://doi.org/10.1099/0022-1317-33-1-61
/content/journal/jgv/10.1099/0022-1317-33-1-61
/content/journal/jgv/10.1099/0022-1317-33-1-61
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