The use of enzyme-linked antibodies for the detection of two morphologically different plant viruses is described. The technique is extremely sensitive, enabling assay of the viruses at concentrations as low as 10 to 100 ng/ml both in purified preparations and in crude plant extracts.
EngvallE.,
PerlmannP.1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
VollerA.,
BidwellD.,
HuldtG.,
EngvallE.1974; A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bulletin of the World Health Organization 51:209–211
VollerA.,
BidwellD. E.,
BartlettA.1976; Enzyme immunoassays in diagnostic medicine: theory and practice. Bulletin of the World Health Organization (in the press)