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Bacteriophage φW-14 is sensitive to osmotic shock. It contains sufficient free putrescine, 2-hydroxyputrescine and spermidine to neutralize about 15% of the DNA phosphates. The α-putrescinylthymine residues of the DNA could neutralize a further 25% of the phosphates. Label is transferred from ornithine to the α-putrescinyl residues of φW-14 DNA. The rates of polyamine synthesis in Pseudomonas acidovorans are increased by φW-14 infection.
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