@article{mbs:/content/journal/jgv/10.1099/0022-1317-32-3-479, author = "Enjuanes, L. and Carrascosa, A. L. and Viñuela, E.", title = "Isolation and Properties of the DNA of African Swine Fever (ASF) Virus", journal= "Journal of General Virology", year = "1976", volume = "32", number = "3", pages = "479-492", doi = "https://doi.org/10.1099/0022-1317-32-3-479", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-32-3-479", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY African swine fever (ASF) virus was grown either in swine macrophages or in VERO cells and purified free of cell DNA. Virus DNA was isolated from virions as a molecule with a sedimentation coefficient of 60S and a contour of 58 ± 3 µm. These two values give a mol. wt. of 102 ± 5 × 106 and 107 ± 5 × 106, respectively, for the genome of ASF virus. Denatured DNA fragments from ASF virus reassociate with a C 0 t ½ value of 0.60 ± 0.05 ms, which compared with the corresponding value for T4 DNA gives for the molecular mass of ASF virus DNA a value of 102 ± 8 × 106 daltons. Only virus DNA is synthesized in ASF virus-infected swine macrophages.", }