1887

Abstract

SUMMARY

Purified preparations of grapevine fanleaf virus consist of three serologically indistinguishable centrifugal components, T, M and B, with sedimentation coefficients of 50, 86 and 120S respectively, and containing 0, 30 and 42% RNA. At equilibrium in CsCl gradients, T, M and B components precipitate. Their buoyant densities are 1.31, 1.41 and 1.49 g/ml, respectively. Infectivity is associated mostly with B component. RNA is single-stranded and occurs as two species with mol. wt. of 1.4 × 10 (RNA-2) and 2.4 × 10 (RNA-1). M particles contain only RNA-2 whereas B particles contain both species. RNA-2 is non-infective, some infectivity is associated with preparations of RNA-1, and infectivity can increase tenfold when RNA-1 and RNA-2 are mixed. The capsid contains a single protein species with mol. wt. of about 54000. Comparable data for arabis mosaic and raspberry mosaic viruses are similar. The present cryptogram of grapevine fanleaf virus is R/1:2.4/42 + 1.4/30:S/S:S/Ne.

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1976-09-01
2022-01-21
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References

  1. AGRA WAL H. O., TREMAINE J. H.1972Proteins of cowpea chlorotic mottle, broad bean mottle and brome mosaic virusesVirology47:8–20
    [Google Scholar]
  2. BACHRACH H. L.1968Foot and mouth disease virusAnnual Review of Microbiology22:201–244
    [Google Scholar]
  3. BARABINO G.1963The identity and relationships of sap-transmissible viruses isolated from Italian grapevinesHorticultural Research3:27–33
    [Google Scholar]
  4. BISHOP D. H. L., CLAYBROOK J. R., SPIEGELMAN S.1967Electrophoretic separation of viral nucleic acids on Polyacrylamide gelsJournal of Molecular Biology26:373–387
    [Google Scholar]
  5. BOATMAN S., KAPER J. M.1972Forces responsible for the generation of virus structures. The use of SDS to probe protein-RNA interactionsProceedings of the First John Innes Symposium123–134Amsterdam: North Holland
    [Google Scholar]
  6. CADMAN C. H., DIAS H. F., HARRISON B. D.1960Sap-transmissible viruses associated with diseases of grapevines in Europe and North AmericaNature, London187:577–579
    [Google Scholar]
  7. CHERVENKA C. H.1969A manual of methods for the analytical ultracentrifugeSpinco Division of Beckman Instruments Inc., Palo AltoCalifornia100
    [Google Scholar]
  8. DIAS H. F., HARRISON B. D.1963The relationship between grapevine fanleaf, grapevine yellow mosaic and arabis mosaic virusesAnnals of Applied Biology51:97–105
    [Google Scholar]
  9. DIENER T. O., SCHNEIDER I. R.1966The two components of tobacco ringspot virus: origin and propertiesVirology29:100–105
    [Google Scholar]
  10. DIENER T. O., SCHNEIDER I. R.1968Virus degradation, and nucleic acid release in single-phase phenol systemsArchives of Biochemistry and Biophysics124:401–412
    [Google Scholar]
  11. HARRISON B. D., FINCH J. T., GIBBS A. J., HOLLINGS M., SHEPHERD R. J., VALENTA V., WETTER C.1971Sixteen groups of plant virusesVirology45:356–363
    [Google Scholar]
  12. HARRISON B. D., MURANT A. F., MAYO M. A.1972aEvidence for two functional RNA species in raspberry ringspot virusJournal of General Virology16:339–348
    [Google Scholar]
  13. HARRISON B. D., MURANT A. F., MAYO M. A.1972bTwo properties of raspberry ringspot virus determined by its smaller RNAJournal of General Virology17:137–141
    [Google Scholar]
  14. HARRISON B. D., MURANT A. F., MAYO M. A., ROBERTS I. M.1974Distribution of determinants for symptom production, host range and nematode transmissibility between the two RNA components of raspberry ringspot virusJournal of General Virology22:233–247
    [Google Scholar]
  15. HEWITT W. B., RASKI D. J., GOHEEN A. C.1958Nematode vector of soil-borne fanleaf virus of grapevinesPhytopathology48:586–595
    [Google Scholar]
  16. HEWITT W. B., MARTELLI G. P., DIAS H. F., TAYLOR R. H.1970Grapevine fanleaf virusC.M.I./A.A.B. Descriptions of Plant Viruses28:4
    [Google Scholar]
  17. JASPARS E. M. J.1974Plant viruses with a multipartite genomeAdvances in Virus Research19:37–149
    [Google Scholar]
  18. MARKHAM R.1967The ultracentrifugeMethods in Virology111Edited byMaramorosch K., Koprowski H.New York and LondonAcademic Press
    [Google Scholar]
  19. MARTELLI G. P.1975Some features of nematode-borne viruses and their relationship with the host plantNematode Vectors of Plant Viruses223Edited byLamberti F., Taylor C. E., Seinhorst J. W.New YorkPlenum Publishing Company
    [Google Scholar]
  20. MARTELLI G. P., HEWITT W. B.1963Purification and serology of Italian strains of grape fanleaf virusPhytopathologia Mediterraneaz:243–253
    [Google Scholar]
  21. MAYO M. A., MURANT A. F., HARRISON B. D.1971New evidence on the structure of nepovirusesJournal of General Virology12:175–178
    [Google Scholar]
  22. MAYO M. A., HARRISON B. D., MURANT A. F., BARKER H.1973Cross-linking of RNA induced by ultraviolet irradiation of particles of raspberry ringspot virusJournal of General Virology19:155–159
    [Google Scholar]
  23. MAYOR H. D., JORDAN L., ITO M.1969Deoxyribonucleic acid of adeno-associated satellite virusJournal of Virology4:191–194
    [Google Scholar]
  24. MURANT A. F., MAYO M. A., HARRISON B. D., GOOLD R. A.1972Properties of virus and RNA components of raspberry ringspot virusJournal of General Virology16:327–338
    [Google Scholar]
  25. QUACQUARELLI A., MARTELLI G. P.1965Studies on Italian and Greek isolates of grapevine fanleaf virusProceedings of the International Conference on Virus and Vector on perennial hosts, with special reference to VitisUniversity of California377–389
    [Google Scholar]
  26. QUACQUARELLI A., PIAZZOLLA P., VOVLAS C.1972aFreezing in the production of artificial top component of chicory yellow mottle virusJournal of General Virology17:147–156
    [Google Scholar]
  27. QUACQUARELLI A., PIAZZOLLA P., VOVLAS C.1972bPreparation of nucleic acid from chicory yellow mottle virusPhytopathologia Mediterranean:207–209
    [Google Scholar]
  28. RAYMER W. B., DIENER T. O.1969Potato spindle tuber virus: a plant virus with properties of a free nucleic acid. I. Assay, extraction and concentrationVirology37:343–350
    [Google Scholar]
  29. REICHMANN M. E.1965Determination of ribonucleic acid content of spherical viruses from sedimentation coefficients of full and empty particlesVirology25:166–169
    [Google Scholar]
  30. SEGHAL O. P., JEAN J. L., BHALLA R. B., SOONG M. M., KRAUSE G. F.1970Correlation between buoyant density and ribonucleic acid content in virusesPhytopathology60:1778–1784
    [Google Scholar]
  31. SINSHEIMER R. L.1959A single-stranded deoxyribonucleic acid from bacteriophage [i]ϕX[/i] 174Journal of Molecular Biology1:43–53
    [Google Scholar]
  32. TAYLOR R. H., HEWITT W. B.1963Properties and serological relationships of Australian and Californian soil-borne viruses of the grapevine and arabis mosaic virusAustralian Journal of Agricultural Research15:571–585
    [Google Scholar]
  33. VAN REGENMORTEL M. H. V.1965Purification of South African isolates of grapevine fanleaf virus by zone electrophoresisProceedings International Conference on Virus and Vector on perennial hosts, with special reference to VitisUniversity of California410–416
    [Google Scholar]
  34. VUITTENEZ A., KUZALA J.1963Isolement, propriétés physiques et sérologiques du virus de la dégénérescence infectieuse chez les chenopodes et la vigneVirologic appliquée4:133–141
    [Google Scholar]
  35. WEBER K., OSBORN M.1969The reliability of molecular weight determinations by dodecyl-sulphate Polyacrylamide gel electrophoresisJournal of Biological Chemistry244:4406–4412
    [Google Scholar]
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