RT Journal Article SR Electronic(1) A1 Cedar, H.YR 1976 T1 Annealing and Hybridization Properties of Herpes Simplex Virus Type 1 DNA JF Journal of General Virology, VO 32 IS 3 SP 337 OP 347 DO https://doi.org/10.1099/0022-1317-32-3-337 PB Microbiology Society, SN 1465-2099, AB SUMMARY The hybridization properties of the herpes simplex virus type 1 (HSV) genome have been analysed. The DNA has a kinetic complexity of 1 × 10-8. E. coli RNA polymerase was found to initiate synthesis at about 70 sites on the HSV DNA. The in vitro RNA product from this reaction was complementary to about 80% of the HSV genome. The RNA-DNA hybridization rate constant (K h ) was determined using conditions of both RNA excess and DNA excess. Using this rate constant one can analyse the content of HSV sequences in any RNA population., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-32-3-337