RT Journal Article SR Electronic(1) A1 Motoyoshi, F. A1 Oshima, N.YR 1976 T1 The Use of Tris-HCl Buffer for Inoculation of Tomato Protoplasts with Tobacco Mosaic Virus JF Journal of General Virology, VO 32 IS 2 SP 311 OP 314 DO https://doi.org/10.1099/0022-1317-32-2-311 PB Microbiology Society, SN 1465-2099, AB SUMMARY When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0.05 m-tris-HCl buffer, pH 8.0, in the presence of poly-l-ornithine or poly-d-lysine, 50 to 80% infection was obtained., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-32-2-311