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The Use of Tris-HCl Buffer for Inoculation of Tomato Protoplasts with Tobacco Mosaic Virus
Abstract
SUMMARY
When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0.05 m-tris-HCl buffer, pH 8.0, in the presence of poly-l-ornithine or poly-d-lysine, 50 to 80% infection was obtained.
- Received:
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© Society for General Microbiology 1976