RNA-Instructed DNA Polymerase Associated with C-Type Particles Produced by Murine Myeloma Cells Free

Abstract

SUMMARY

C-type particles secreted by MOPC-315 myeloma cells were characterized. These particles localize at a density of 1.16 g/ml in sucrose and possess a 60 to 70S RNA and an RNA-instructed DNA polymerase. Endogenous enzyme activity requires manganese and is inhibited by ribonuclease or by the omission of any of the deoxynucleoside triphosphates. The enzyme utilizes the virus 60 to 70S RNA as a template to synthesize DNA molecules which specifically hybridize to the homologous RNA.

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1976-08-01
2024-03-28
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References

  1. DALTON A. J., POTTER M., MERWIN R. M. 1961; Some ultrastructural characteristics of a series of primary and transplanted plasma cell tumors of the mouse. Journal of the National Cancer Institute 26:1221–1267
    [Google Scholar]
  2. GOODMAN V. C., SPIEGELMAN S. 1971; Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proceedings of the National Academy of Sciences of the United States of America 68:2203–2206
    [Google Scholar]
  3. KARPAS A., CAWLEY J. 1972a; Isolation of a C-type virus from mouse myeloma cells. European Journal of Cancer 8:363–364
    [Google Scholar]
  4. KARPAS A., CAWLEY J. 1972b; A and C-type particles of mouse myeloma cells. European Journal of Cancer 8:601–604
    [Google Scholar]
  5. KUFF E. L., WIVEL N. A., LUEDERS K. K. 1968; The extraction of intracisternal A-particles from a mouse plasma-cell tumor. Cancer Research 28:2137–2148
    [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH V. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  7. MERWIN R. M., REDMON L. W. 1963; Induction of plasma cell tumours and sarcomas in mice by diffusion chambers placed in the peritoneal cavity. Journal of the National Cancer Institute 31:997–1017
    [Google Scholar]
  8. POTTER M., LIEBERMAN R. 1967; Genetics of immunoglobulins in the mouse. Advances in Immunology 7:91–145
    [Google Scholar]
  9. SCHLOM J., SPIEGELMAN S. 1971; Simultaneous detection of reverse transcriptase and high molecular RNA unique to oncogenic RNA viruses. Science, New York 174:840–844
    [Google Scholar]
  10. SCHUBERT D., HORIBATA K. 1968; Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. Journal of Molecular Biology 38:263–271
    [Google Scholar]
  11. TEMIN H. M., BALTIMORE D. 1972; RNA-directed DNA synthesis and RNA tumor viruses. Advances in Virus Research 17:129–186
    [Google Scholar]
  12. VOLKMAN L. E., KRUEGER R. G. 1973a; XC cell cytopathogenicity as an assay for murine myeloma C-type virus. Journal of the National Cancer Institute 51:1205–1210
    [Google Scholar]
  13. VOLKMAN L. E., KRUEGER R. G. 1973b; Characterization of C-type particles produced by a tissue culture-adapted murine myeloma. Journal of Virology 12:1589–1597
    [Google Scholar]
  14. WATSON J., RALPH P., SARKAR S., COHN M. 1970; Leukemia viruses associated with mouse myeloma cells. Proceedings of the National Academy of Sciences of the United States of America 66:344–351
    [Google Scholar]
  15. YANIV A., GULATI S. C, BURNY A., SPIEGELMAN S. 1973; Detection of complexes containing 70S RNA and reverse transcriptase in human leukemic plasma. Intervirology 1:317–328
    [Google Scholar]
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