@article{mbs:/content/journal/jgv/10.1099/0022-1317-32-2-295, author = "Chanas, A. C. and Johnson, B. K. and Simpson, D. I. H.", title = "Antigenic Relationships of Alphaviruses by a Simple Micro-culture Cross-neutralization Method", journal= "Journal of General Virology", year = "1976", volume = "32", number = "2", pages = "295-300", doi = "https://doi.org/10.1099/0022-1317-32-2-295", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-32-2-295", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY A cross-neutralization study of 22 alphaviruses disclosed two major antigenic complexes and two viruses that were distinct from all the others. Cross-reactions were common but were restricted within the complexes. Sub-groupings within a complex were also shown, and some viruses proved to be indistinguishable by neutralization testing. The results generally paralleled previously reported data. Investigations were carried out, using BHK 21 cells in a micro tissue-culture system, on all available alphaviruses.", }