1887

Abstract

SUMMARY

The Xf phage coat protein associated with infected cells could not be removed by washing with antiserum and tris-EDTA buffer. Although the infected cells were consecutively washed 6 times with tris-EDTA buffer, the ratios of parental phage H-DNA to C-protein were not changed. A considerable amount of the parental C-protein and H-DNA in the original ratio were detected in the membrane and the soluble cytoplasmic fractions of infected cells. The studies of the change in Xf C-protein and H-DNA incorporation into the host cells and their release showed that DNA and protein penetrate together into the host cells during the first 60 min after infection (p.i.). While virtually all parental DNA was conserved, re-utilized and released from the infected cell 60 min p.i., no apparent release of parental protein was observed. Approx. 40% of the parental protein became degraded and could be washed from the infected cell after 90 min; the rest of the parental protein remained and probably was re-utilized by the host.

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/content/journal/jgv/10.1099/0022-1317-32-2-241
1976-08-01
2021-10-23
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References

  1. BRADLEY D. E. 1973; The adsorption [i]of Pseudomonas aeruginosa[/i] filamentous bacteriophage Pf to its host. Canadian Journal of Microbiology 19:623–631
    [Google Scholar]
  2. CARO L. G., SCHNÖS M. 1966; The attachment of the male-specific bacteriophage fi to sensitive strains of [i]E. coli[/i]. Proceedings of National Academy of Sciences of the United States of America 56:126–132
    [Google Scholar]
  3. CHRISTENSE H. R., TOLMACH L. J. 1955; On the early stages of infection of [i]Escherichia coli[/i] B by bacteriophage T1. Archives of Biochemistry and Biophysics 57:195–207
    [Google Scholar]
  4. CRAWFORD E. M., GESTELAND R. F. 1964; The adsorption of bacteriophage R17. Virology 2:165–167
    [Google Scholar]
  5. EDGELL M. H., GINOZA W. 1965; The fate during infection of the coat protein of the spherical bacteriophage R17. Virology 27:23–27
    [Google Scholar]
  6. HENRY T. J., JUN. BRINTON C. C. 1971; Removal of the coat protein of bacteriophages M13 or fd from the exterior of the host after infection. Virology 46:754–763
    [Google Scholar]
  7. KUO T. T., HUANG T. C., CHOW T. Y. 1969; A filamentous bacteriophage from [i]Xanthomonas oryzae[/i]. Virology 39:548–555
    [Google Scholar]
  8. MEYNELL G. G., LAWN A. M. 1968; Filamentous phage specific for the 1 sex factor. Nature, London 217:1184–1186
    [Google Scholar]
  9. NEWBOLD J. E., SINSHEIMER R. L. 1970; The process of infection with bacteriophage [i]ϕ[/i]X 174. Early steps in the infection process: attachment, eclipse and DNA penetration. Journal of Molecular Biology 49:49–66
    [Google Scholar]
  10. SIMON L. D., ANDERSON T. F. 1967; The infection of [i]Escherichia coli[/i] by T2 and T4 bacteriophages as seen in the electron microscope 1. Attachment and penetration. Virology 32:279–297
    [Google Scholar]
  11. TRENKNER E., BONHOEFFER F., GIERER A. 1967; The fate of the protein component of bacteriophage fd during infection. Biochemical and Biophysical Research Communications 28:932–939
    [Google Scholar]
  12. TZAGOLOFF H., PRATT D. 1964; The initial steps in infection with coliphage M13. Virology 24:372–380
    [Google Scholar]
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