1887

Abstract

SUMMARY

Interferon inhibits the replication of simian virus 40 (SV40) in monkey cells and reduces markedly the formation of both early virus protein (i.e. SV40 T antigen) and early SV40 RNA. This suggests that in SV40 infection interferon acts primarily by inhibiting transcription. To test this conclusion further, we examined alternative mechanisms which might explain these results and made the following observations. (1) The quantity of input SV40 DNA in the nucleus 24 h post infection (p.i.) was the same in interferon-treated and control cells. Thus interferon does not appear to diminish the quantity of SV40 DNA template available for transcription. (2) Chemical inhibitors of protein synthesis did not mimic the selective inhibition of early SV40 RNA formation induced by interferon, indicating that the transcription of early SV40 RNA is not dependent upon the prior synthesis of any virus-induced protein. Thus a block in translation cannot readily explain the reduced formation of early SV40 RNA in interferon-treated cells. (3) Fractionation of SV40 infected cells after a one-hour labelling period showed that interferon produced a comparable reduction in the quantity of early SV40 RNA in the nucleus and the cytoplasm. Thus the observed inhibition of early SV40 RNA is not due solely (if at all) to enhanced cytoplasmic degradation. These results indicate that the primary effect of interferon in SV40 infected monkey cells is either to inhibit the transcription of early virus RNA or to enhance its turnover in the nucleus.

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1976-08-01
2022-01-29
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References

  1. ALOM Y.1972Extensive symmetrical transcription of simian virus 40 DNA in virus-yielding cellsProceedings of the National Academy of Sciences of the United States of America69:2404–2409
    [Google Scholar]
  2. BEAN W. J., SIMPSON R. W.1973Primary transcription of the influenza virus genome in permissive cellsVirology56:646–651
    [Google Scholar]
  3. BEARD P., ACHESON N. H., MAXWELL I. H.1976Strand-specific transcription of polyoma virus DNA early in productive infection and in transformed cellsJournal of Virology17:20–26
    [Google Scholar]
  4. BIALY H. S., COLBY C.1972Inhibition of early vaccinia virus ribonucleic acid synthesis in interferon-treated chicken embryo fibroblastsJournal of Virology9:286–289
    [Google Scholar]
  5. BILLIAU A., SOBIS H., DE SOMER P.1973Influence of interferon on virus particle formation in different oncornavirus carrier cell linesInternational Journal of Cancer12:646–653
    [Google Scholar]
  6. BODO G., SCHEIRER W., SUH M., SCHULTZE B., HORAK I., JUNGWIRTH C.1972Protein synthesis in pox-infected cells treated with interferonVirology50:140–147
    [Google Scholar]
  7. BROWN A. H.1946Determination of pentose in the presence of large quantities of glucoseArchives of Biochemistry11:269–278
    [Google Scholar]
  8. BURTON K.1956A study of the conditions and mechanism of the diphenylamine reaction for the colori-metric estimation of deoxyribonucleic acidBiochemical Journal62:315–323
    [Google Scholar]
  9. DARNELL J. E., JELINEK W. R., MOLLOY G. R.1973Biogenetics of mPvNA: genetic regulation in mammalian cellsScience, New York181:1215–1221
    [Google Scholar]
  10. ESTEBAN R. M., METZ D. H.1973Inhibition of early vaccinia virus protein synthesis in interferon-treated chick embryo fibroblastsJournal of General Virology20:111–115
    [Google Scholar]
  11. FRIEDMAN T., HAAS M.1970Rapid concentration and purification of polyoma virus and SV40 with polyethylene glycolVirology42:248–250
    [Google Scholar]
  12. FRIEDMAN B., RAMSEUR J. M.1974Inhibition of murine leukemia virus production in chronically infected AKR cells; a novel effect of interferonProceedings of the National Academy of Sciences of the United States of America71:3542–3544
    [Google Scholar]
  13. GILLESPIE D., SPIEGELMAN S.1965A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology12:829–842
    [Google Scholar]
  14. GRAESSMAN A., GRAESSMAN M., HOFFMANN H., NIEBEL J., BRANDNER G., MUELLER N.1974Inhibition by interferon of SV40 tumor antigen formation in cells injected with SV40 cRNA transcribed [i]in vitro[/i]FEBS Letters39:249–251
    [Google Scholar]
  15. GRAVELL J., CROMEANS T.1972Inhibition of early viral RNA synthesis in interferon treated cells infected with frog polyhedral cytoplasmic deoxyribovirusVirology50:916–919
    [Google Scholar]
  16. JOKLIK W. K., MERIGAN T. C.1966Concerning the mechanism of action of interferonProceedings of the National Academy of Sciences of the United States of America56:558–565
    [Google Scholar]
  17. JUNGWIRTH C, HORAK I., BODO O., LINDNER J., SCHULTZE B.1972The synthesis of pox virus-specific RNA in interferon-treated cellsVirology48:59–70
    [Google Scholar]
  18. KAMEN R., LINDSTROM D. M., SHURE H., OLD R. W.1974Virus-specific RNA in cells productively infected or transformed by polyoma virusCold Spring Harbor Symposium on Quantitative Biology39:187–198
    [Google Scholar]
  19. LEVIN M. J., OXMAN M. N., DIAMANDOPOULOS G. TH., LEVINE A. S., HENRY P. H., ENDERS J. F.1969Virus-specific nucleic acids in SV40 exposed hamster embryo cell lines: correlation with S and T antigensProceedings of the National Academy of Sciences of the United States of America62:589–596
    [Google Scholar]
  20. LEVINE A. S., OXMAN M. N., LEVIN M. J., HENRY P. H., DIAMANDOPOULOS G. TH., ENDERS J. F.1970Virus-specific deoxyribonucleic acid in simian virus 40-exposed hamster cells: correlation with S and T antigensJournal of Virology6:199–207
    [Google Scholar]
  21. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J.1951Protein measurement with the Folin phenol reagentsJournal of Biological Chemistry193:265–275
    [Google Scholar]
  22. MANDERS E. K., TILLES J. G., HUANG A. S.1972Interferon-mediated inhibition of virion-directed transcriptionVirology49:573–581
    [Google Scholar]
  23. MARCUS P. I., ENGELHARDT D. L., HUNT J. M., SEKELLICK M.J.1971Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymeraseScience, New York174:593–598
    [Google Scholar]
  24. MARCUS P. I., TERRY T. M., LEVINE S.1975Interferon action. II. Membrane-bound alkaline ribonuclease activity in chick embryo cells manifesting interferon-mediated interferenceProceedings of the National Academy of Sciences of the United States of America72:182–186
    [Google Scholar]
  25. MARMUR J.1961A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology3:208–218
    [Google Scholar]
  26. METZ D. H.1975Discrimination between viral and cellular macromolecular synthesisControl Processes - Virus Multiplication25th Symposium of the Society for General Microbiology323–353Edited byBurke D. C., Russell W. C.
    [Google Scholar]
  27. METZ D. H., ESTEBAN R. M.1972Interferon inhibits viral protein synthesis in L cells infected with vaccinia virusNature, London238:385–388
    [Google Scholar]
  28. METZ D. H., ESTEBAN R. M., DANIELESCU G.1975The effect of interferon on the formation of virus polyribosomes in L cells infected with vaccinia virusJournal of General Virology27:197–210
    [Google Scholar]
  29. NYGAARD A. P., HALL B. D.1963A method for the detection of RNA-DNA complexesBiochemical and Biophysical Research Communications12:98–104
    [Google Scholar]
  30. OXMAN M. N., BLACK P. H.1966Inhibition of SV40 T antigen formation by interferonProceedings of the National Academy of Sciences of the United States of America55:1133–1140
    [Google Scholar]
  31. OXMAN M. N., LEVIN M. J.1971Interferon and transcription of early virus-specific RNA in cells infected with simian virus 40Proceedings of the National Academy of Sciences of the United States of America68:299–302
    [Google Scholar]
  32. OXMAN M. N., LEVINE A. S., CRUMPACKER C. S., LEVIN M. J., HENRY P. H., LEWIS A. M.1971Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. IV. Characterization of the simian virus 40 ribonucleic acid species induced by wild-type simian virus 40 and by the nondefective hybrid virus Ad2[sup]+[/sup]ND[sub]-[/sub]Journal of Virology8:215–224
    [Google Scholar]
  33. OXMAN M. N., LEVIN M. J., JUN. LEWIS A. M.1974Control of SV40 gene expression in adenovirus-simian virus 40 hybrid viruses: the synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virusJournal of Virology13:322–330
    [Google Scholar]
  34. OXMAN M. N., ROWE W. P., BLACK P. H.1967Studies of adenovirus-SV4o hybrid viruses. VI. Differential effects of interferon on SV40 and adenovirus T antigen formation in cells infected with SV40 virus, adenoviruses, and adenovirus-SV40 hybrid virusesProceedings of the National Academy of Sciences of the United States of America57:941–948
    [Google Scholar]
  35. PENMAN S.1969Preparation of purified nucleic acid and nucleoli from mammalian cellsFundamental Techniques in Virology35–48Edited byHabel K., Salzman N. P.New YorkAcademic Press
    [Google Scholar]
  36. POPE J. H., ROWE W. P.1964Detection of specific antigens in SV40 transformed cells by immunofluorescenceJournal of Experimental Medicine120:121–128
    [Google Scholar]
  37. REPIK P., FLAMAND A., BISHOP D. H. L.1974Effect of interferon upon the primary and secondary transcription of vesicular stomatitis and influenza virusesJournal of Virology14:1169–1178
    [Google Scholar]
  38. SAMUEL C. E., JOKUK W. K.1974A protein synthesizing system from interferon-treated cells that discriminates between cellular and viral messenger RNAsVirology58:476–491
    [Google Scholar]
  39. SCHNEIDER W. C.1945Phosphorus compounds in animal tissues. 1. Extraction and estimation of desoxy-pentose nucleic acid and of pentose nucleic acidJournal of Biological Chemistry161:293–303
    [Google Scholar]
  40. SINGER R. H., PENMAN S.1972Stability of HeLa cell mRNA to actinomycinNature, London240:100–102
    [Google Scholar]
  41. SNEDECOR G. W., COCHRAN W. G.1973Statistical Methods6th ed.549Ames, Iowa: The Iowa State University Press
    [Google Scholar]
  42. STRANDER H., CANTELL K.1967Further studies on the production of interferon by human leukocytes [i]in vitro[/i]Annales Medicinae Experimentalis et Biologiae Fenniae45:20–27
    [Google Scholar]
  43. WEINBERG R. A., WARNAAR S. O., WINOCOUR E.1972Isolation and characterization of simian virus 40 ribonucleic acidJournal of Virology10:193–201
    [Google Scholar]
  44. YAMAMOTO K., YAMAGUCHI N., ODA K.1975Mechanism of interferon-induced inhibition of early simian virus 40 (SV40) functionsVirology68:58–70
    [Google Scholar]
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