Skip to content
1887

Abstract

SUMMARY

The surface nature of the proteins of a bovine enterovirus have been determined by using I and pyridoxal phosphate-sodium borohydride labelling techniques. As found previously, I labels only VP in intact capsid particles, whereas reaction with pyridoxal phosphate followed by reduction with tritiated sodium borohydride labels VP, VP and VP. Only VP is found to have no surface tyrosine, histidine or lysine available for reaction. After neutralization with homologous antisera, however, VP becomes exposed and is then available for labelling with I. This must reflect a substantial conformational change in the virus particle after neutralization.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-32-1-17
1976-07-01
2025-04-29
Loading full text...

Full text loading...

References

  1. BREESE S. S., HSU K. C.1971Methods in Virology5404London: Academic Press
    [Google Scholar]
  2. BREINDL M.1971The structure of heated poliovirus particlesJournal of General Virology11:147–156
    [Google Scholar]
  3. CARTHEW P., MARTIN S. J.1974The iodination of bovine enterovirus particlesJournal of General Virology24:525–534
    [Google Scholar]
  4. DULBECCO R., VOGT M., STRICKLAND A. G. R.1956A study of the basic aspects of neutralisation of two animal viruses, western equine encephalitis virus and poliomyelitis virusVirology2:162–205
    [Google Scholar]
  5. HOEY E. M., MARTIN S. J.1974A possible precursor containing RNA of a bovine enterovirus: the provirion IIJournal of General Virology24:515–524
    [Google Scholar]
  6. JOHNSTON M. D., MARTIN S. J.1971Capsid and procapsid proteins of a bovine enterovirusJournal of General Virologyn:71–79
    [Google Scholar]
  7. MACPHERSON I. A., STOKER M.1962Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competenceVirology16:147–151
    [Google Scholar]
  8. MANDEL B.1971Characterisation of type I poliovirus by electrophoretic analysisVirology44:554–568
    [Google Scholar]
  9. MARTIN S. J., JOHNSTON M. D., CLEMENTS J. B.1970Purification and characterization of bovine enterovirusesJournal of General Virology7:103–113
    [Google Scholar]
  10. RIFKIN D. B., COMPANS R. W., REICH E.1972A specific labelling procedure for proteins on the outer surface of membranesJournal of Biological Chemistry247:6432–6437
    [Google Scholar]
  11. TALBOT P., ROWLANDS D. J., BURROUGHS J. N., SANGAR D. V., BROWN F.1973Evidence for a group protein in foot-and-mouth disease virusJournal of General Virology19:369–380
    [Google Scholar]
  12. TALBOT D. V., YPHANTIS D. A.1971Fluorescent monitoring of SDS gel electrophoresisAnalytical Biochemistry44:246–253
    [Google Scholar]
  13. WALLIS C., MELNICK J. L.1967Virus aggregation as the cause of the non-neutralizable persistent fractionJournal of Virologyi:478–488
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-32-1-17
Loading
/content/journal/jgv/10.1099/0022-1317-32-1-17
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error