1887

Abstract

SUMMARY

The sedimentation rate of human leukocyte interferon (HLIF) reactivated from sodium dodecyl sulphate (SDS) solution was studied by glycerol gradient centrifugation and compared to that of native HLIF. Reactivated HLIF consistently sedimented faster than native HLIF, indicating that full recovery of antiviral activity does not require renaturation of the entire interferon molecule.

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1976-07-01
2022-06-29
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References

  1. CASTELLINO F. S., BARKER R. 1968; Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry. Biochemistry 7:2207–2217
    [Google Scholar]
  2. DAYHOFF M. O., ECK R. V. 1967-1968 Atlas of protein sequence and structure. National Biomedical Research Foundation, Silver Spring; Maryland:
    [Google Scholar]
  3. DESMYTER J., STEWART W. E. II 1976; Molecular modification of interferon: attainment of human interferon active on heterologous cells without activity on homologous cells. Virology in the press
    [Google Scholar]
  4. FANTES K. H. 1970; Purification and properties of human interferon. Annals of the New York Academy of Sciences 173:118–126
    [Google Scholar]
  5. HAVELL E. A., VILCEK J. 1972; Production of high titered interferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  6. JUN. KNIGHT E. 1975; Heterogeneity of purified mouse interferons. Journal of Biological Chemistry 250:4139–4143
    [Google Scholar]
  7. JUN. KNIGHT E. 1976; Interferon: purification and initial characterization from human diploid cells. Proceedings of the National Academy of Sciences of the United States of America in the press
    [Google Scholar]
  8. JUN. MAIZEL J. V. 1971 Methods in Virology 5179–246 Edited by K. & H. Koprowski New York: Academic Press;
    [Google Scholar]
  9. MARTIN R. G., AMES B. V. 1961; A method for determining the sedimentation behavior of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  10. REYNOLDS F., PITHA P. 1975; Characterization of human interferon produced in cell-free protein synthesis system. Biochemical and Biophysical Research Communications 65:107–115
    [Google Scholar]
  11. STEWART W. E. II 1974; Distinct molecular species of interferons. Virology 61:80–86
    [Google Scholar]
  12. STEWART W. E. II, DE CLERCQ E., DE SOMER P. 1974; Stabilization of interferons by defensive reversible denaturation. Nature, London 349:460–461
    [Google Scholar]
  13. STEWART W. E. II, DESMYTER J. 1975; Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weight, requirements for renaturation, and cross-species antiviral activity. Virology 67:68–73
    [Google Scholar]
  14. STEWART W. E. II, DE SOMER P., DE CLERCQ E. 1974; Renaturation of inactivated interferon: requirement for reduction of a minor component; lack of requirement for reduction of a minor component. Journal of General Virology 24:567–570
    [Google Scholar]
  15. STEWART W. E. II, DE SOMER P., EDY V. G., PAUCKER K., BERG K., OGBURN C. A. 1975; Distinct molecular species of human interferons: requirements for stabilization and reactivation of human leukocyte and fibroblast interferon. Journal of General Virology 26:327–330
    [Google Scholar]
  16. TANFORD C, KAWAHARA K., LAPANJE S. 1967; Proteins as random coils. I. Intrinsic viscosity and sedimentation coefficients in concentrated guanidine hydrochloride. Journal of the American Chemical Society 89:729–736
    [Google Scholar]
  17. WEBER K., KUTER D. J. 1971; Reversible denaturation of enzymes by sodium dodecyl sulfate. Journal of Biological Chemistry 246:4504–4509
    [Google Scholar]
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