Evidence that Detergent-reactivated Interferons are not Renatured

P. T. Allen; W. E. Stewart II

doi:10.1099/0022-1317-32-1-133

Evidence that Detergent-reactivated Interferons are not Renatured

P. T. Allen and W. E. Stewart II
First Published: 01 July 1976 https://doi.org/10.1099/0022-1317-32-1-133

Abstract

Summary

The sedimentation rate of human leukocyte interferon (HLIF) reactivated from sodium dodecyl sulphate (SDS) solution was studied by glycerol gradient centrifugation and compared to that of native HLIF. Reactivated HLIF consistently sedimented faster than native HLIF, indicating that full recovery of antiviral activity does not require renaturation of the entire interferon molecule.

Received: 13/08/1975
Accepted: 11/03/1976
Published Online: 01/07/1976

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Journal of General Virology

Volume 32, Issue 1

Research Article

Free

Evidence that Detergent-reactivated Interferons are not Renatured

Abstract

Most read this month

Most cited this month Most Cited RSS feed

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Mechanisms and enzymes involved in SARS coronavirus genome expression

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ICTV Virus Taxonomy Profile: Flaviviridae

SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

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Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures