1887

Abstract

SUMMARY

Focus formation by Rous sarcoma virus (RSV) was significantly enhanced when virus was incubated with the saline fraction of chloroform extracted chicken egg yolk, prior to infecting chicken embryo cells. The enhancement was restricted to members of RSV subgroups B, C and D and was proportional to yolk dilution. Subgroup A virus was never affected. In all, 108 yolk samples from specific pathogen free chickens were investigated. Of these 78 to 97% stimulated focus formation. RSV(RAV-50) of subgroup D which was stimulated up to tenfold, was the most strongly affected strain. The enhancing principle was shown to be a specific yolk factor, and its effect remained constant even after several years' storage. Crude yolk specimens showed essentially the same enhancing property. The chemical nature of the yolk factor is still unknown. It must, however, be taken into account when assaying for avian leukosis virus antibodies.

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1976-07-01
2022-01-23
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References

  1. AULISIO C. G., SHELOKOV A.1967Substitution of egg yolk for serum in indirect fluorescence assay for Rous sarcoma virus antibodyProceedings of the Society of Experimental Biology and Medicine136:312–315
    [Google Scholar]
  2. HANAFUSA T., HANAFUSA H.1967Interaction among avian tumor viruses giving enhanced infectivityProceedings of the National Academy of Sciences of the United States of America58:818–825
    [Google Scholar]
  3. KASS S. J., LEVINSON W.1974Enhancing factor for Rous sarcoma virus: [i]in vitro[/i] assay and action on both cells and virusVirology57:291–295
    [Google Scholar]
  4. KOTTARIDIS S. D., CHOMIAK T. W., LUOINBUHL R. E.1967Compared antibodies for Rous sarcoma virus in chicken serum and egg yolk with the metabolism inhibition testAvian Diseases11:65–68
    [Google Scholar]
  5. PRINCE A. M.1962Factors influencing the determination of cellular morphology in cells infected with Rous sarcoma virusVirology18:524–534
    [Google Scholar]
  6. RAUCH W.1968Handbuch der LebensmittelchemieIll886–890Edited byKiermeier F.Berlin, Heidelberg, New York: Springer-Verlag
    [Google Scholar]
  7. RUBIN H., CORNELIUS A., FANSHIER L.1961The pattern of congenital transmission of an avian leukosis virusProceedings of the National Academy of Sciences of the United States of America47:1058–1069
    [Google Scholar]
  8. RUBIN H., FANSHIER L., CORNELIUS A., HUGHES W. F.1962Tolerance and immunity in chickens after congenital and contact infection with avian leukosis virusVirology17:143–156
    [Google Scholar]
  9. RUOSLAHTI E., VAHERI A., ESTOLA T., SANDELIN K.1973Antibodies against avian gs antigen in chickens infected naturally and experimentally with avian RNA tumor virusesInternational Journal of Cancer11:596–603
    [Google Scholar]
  10. RUSSELL W. C, NEWMAN C., WILLIAMSON D. H.1975A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and virusesNature, London253:461–462
    [Google Scholar]
  11. SANDELIN K., ESTOLA T.1970Studies on the possibility of obtaining leukosis-free eggs from commercial poultry flocks with reference to current laboratory methodsActa Pathologica Microbiologica Scandi-navicaB78:473–478
    [Google Scholar]
  12. SANDELIN K., ESTOLA T.1974Occurrence of different subgroups of avian leukosis virus in Finnish poultryAvian Pathology3:159–168
    [Google Scholar]
  13. SANDEÜN K., ESTOLA T., RISTIMÁKI S., RUOSLAHTI E., VAHERI A.1974Radioimmunoassay of the group-specific antigen in detection of avian leukosis virus infectionJournal of General Virology25:415–420
    [Google Scholar]
  14. SANDELIN K., ESTOLA T.1975Testing and management of a specific pathogen free chicken flock with special reference to avian leukosis virus infectionsActa Veterinaria Scandinavica16:341–356
    [Google Scholar]
  15. SUNI J., VAHERI A., RUOSLAHTI E.1973Radioimmunoassay of RNA tumor virus group-specific antigenIntervirology1:119–126
    [Google Scholar]
  16. TOYOSHIMA K., VOGT P. K.1969Enhancement and inhibition of avian sarcoma viruses by polycations and polyanionsVirology38:414–426
    [Google Scholar]
  17. VAHERI A., RUOSLAHTI E.1973Expression of the major group-specific antigen (gs-a) of avian type-C viruses in normal chicken cells and tissuesInternational Journal of Cancer12:361–367
    [Google Scholar]
  18. VIGIER P.1973Effect of nonspecific factors on focus formation by Rous sarcoma virus. II. Polyanions, divalent cations, temperature and tryptose phosphate brothIntervirology1:338–347
    [Google Scholar]
  19. VOGT P. K.1967DEAE-dextran: enhancement of cellular transformation induced by avian sarcoma virusesVirology33:175–177
    [Google Scholar]
  20. WEISS R.1969Interference and neutralization studies with Bryan strain Rous sarcoma virus synthesized in the absence of helper virusJournal of General Virology5:529–539
    [Google Scholar]
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