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Using 5′-nucleotidase and NADPH:cytochrome c reductase as respective enzyme markers for the plasma membrane and endoplasmic reticulum, a satisfactory separation of these two membrane fractions from a cell line (SMB) derived from a scrapie mouse brain has been achieved. The coincident distribution of scrapie infectivity and 5′-nucleotidase in various fractions isolated from these cells indicates that most of the scrapie infectivity present in this cell line is associated with the plasma membrane.
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