1887

Abstract

SUMMARY

The purified fibre and hexon of adenovirus 12 inhibit the transformation in tissue culture of murine sarcoma virus (MSV-M) by as much as 80% and 70%, respectively, when they are added to cells 8 to 20 h before MSV-M infection. During a 12 h period, only about 6 to 8% of added radiolabelled viral proteins become associated with cells (or 1·0 µg protein bound/10 cells). No inhibition occurs when the proteins are added simultaneously with MSV-M or 90 min or 4 h after MSV-M. There is also a direct correlation between the extent of focus inhibition and the concentrations of viral proteins used. Concentrations of viral proteins used to inhibit cell transformation do not affect cell growth, but do reduce cell macromolecular synthesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-3-437
1976-06-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/3/JV0310030437.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-3-437&mimeType=html&fmt=ahah

References

  1. ABID S. H., KHOOBYARIAN N. 1976; Antitumour activity of adenovirus 12 structural proteins against Moloney sarcoma tumours in mice. British Journal of Cancer in the press
    [Google Scholar]
  2. LEVINE J. A., GINSBERG H. S. 1967; Mechanism by which fiber antigen inhibits multiplication of type 5 adenovirus. Journal of Virology 1:747–757
    [Google Scholar]
  3. LEVINE A. J., GINSBERG H. S. 1968; Role of adenovirus structural proteins in the cessation of host-cell biosynthetic functions. Journal of Virology 2:430–439
    [Google Scholar]
  4. LONG W. K. JUN., KHOOBYARIAN N. 1973; Effect of adenovirus soluble antigens on virus synthesis and transformation in diploid cells. Journal of the National Cancer Institute 51:1527–1533
    [Google Scholar]
  5. MOLONEY J. B. 1956; Biological studies on the murine sarcoma virus. V. Preparation of improved standard lots of the virus for use in quantitative investigation. Journal of the National Cancer Institute 16:887–888
    [Google Scholar]
  6. PHILLIPS D. R., MORRISON M. 1970; The arrangement of proteins in the human erythrocyte membrane. Biochemical and Biophysical Research Communications 40:284–289
    [Google Scholar]
  7. REISFELD R. A., SMALL P. A. JUN. 1966; Electrophoretic heterogeneity of polypeptide chains of specific antibodies. Science, New York 152:1253–1255
    [Google Scholar]
  8. SHATKIN A. J. 1969 Estimation of RNA, DNA, and protein by the use of isotopic precursors followed by chemical fractionation. Fundamental Techniques in Virology238–241 New York: Academic Press, Inc;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-3-437
Loading
/content/journal/jgv/10.1099/0022-1317-31-3-437
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error