1887

Abstract

SUMMARY

Protein synthesis in cucumber cotyledons infected with two strains of cucumber mosaic virus (CMV) differing in symptom type was investigated by the technique of radioactive double-labelling of polypeptides followed by analysis on SDS polyacrylamide gels. Radioactive labelling in the presence of actinomycin D revealed several virus-stimulated proteins associated with infection. In addition to coat protein, three polypeptides with apparent mol. wt. larger than that of coat protein, together with two smaller species were detected. No qualitative difference was observed in the patterns of protein stimulation which might account for the difference in symptoms induced by the two strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-3-373
1976-06-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/3/JV0310030373.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-3-373&mimeType=html&fmt=ahah

References

  1. BARBARA D. J., WOOD K. R. 1974; The influence of actinomycin D on CMV (strain W) multiplication in cucumber cultivars. Physiological Plant Pathology 4:45–50
    [Google Scholar]
  2. BUTTERWORTH B. E. 1973; A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus-iA, and poliovirus. Virology 56:439–453
    [Google Scholar]
  3. CLARK G. L., PEDEN K. W. C., SYMONS R. H. 1974; Cucumber mosaic virus-induced RNA polymerase: partial purification and properties of the template-free enzyme. Virology 62:434–443
    [Google Scholar]
  4. COOPER P. D., STEINER-PRYOR A., WRIGHT P. J. 1973; A proposed regulator for poliovirus: the equestron. Intervirology 1:1–10
    [Google Scholar]
  5. DAVIES J. W., KAESBERG P. 1974; Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ. Journal of General Virology 25:11–20
    [Google Scholar]
  6. EFRON D., MARCUS A. 1973; Translation of TMV-RNA in a cell-free wheat embryo system. Virology 53:343–348
    [Google Scholar]
  7. GARWES D., SILLERO A., OCHOA S. 1969; Virus-specific proteins in E. coli infected with phage Q. Biochimica et Biophysica Acta 186:166–172
    [Google Scholar]
  8. HABILI N., FRANCKI R. I. B. 1974; Comparative studies on tomato aspermy and cucumber mcsaic viruses. III. Further studies on relationship and construction of a virus from parts of the two viral genomes. Virology 61:443–449
    [Google Scholar]
  9. HARIHARASUBRAMANIAN V., HADIDI A., SINGER B., FRAENKEL-CONRAT H. 1973; Possible identification of a protein in brome mosaic virus infected barley as a component of viral RNA polymerase. Virology 54:190–198
    [Google Scholar]
  10. IANDOLO J. J. 1970; Device for slicing Polyacrylamide gels. Analytical Biochemistry 36:6–10
    [Google Scholar]
  11. JONES J. M., REICHMANN M. E. 1973; The proteins synthesized in tobacco leaves infected with tobacco necrosis virus and satellite tobacco necrosis virus. Virology 52:49–56
    [Google Scholar]
  12. KAPER J. M., WEST C. K. 1972; Polyacrylamide gel separation and molecular weight determination of the components of cucumber mosaic virus RNA. Preparative Biochemistry 2:251–263
    [Google Scholar]
  13. KNOWLAND J. 1974; Protein synthesis directed by the RNA from a plant virus in a normal animal cell. Genetics 78:383–394
    [Google Scholar]
  14. LOT H., MARCHOUX G., MARROU J., KAPER J. M., WEST C K., VAN VLOTEN-DOTING L., HULL R. 1974; Evidence for three functional RNA species in several strains of cucumber mosaic virus. Journal of General Virology 22:81–93
    [Google Scholar]
  15. MAKEL J. V. JUN. 1966; Acrylamide gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science, New York 151:988–990
    [Google Scholar]
  16. MARCHOUX G., DEVERGNE J. C, MARROU J., DOUINE L., LOT H. 1974; Complémentation entre ARN de différentes souches du virus de la mosaïque du concombre. Localisation sur l’ARN 3 de plusieurs propriétés, dont certaines liées à la nature de la capside. Comptes Rendu Hebdomadaires des Séances de Γ Académie des Sciences, Paris D 279:2165–2168
    [Google Scholar]
  17. MAYOL R. F., SINSHEIMER R. L. 1970; Process of infection with bacteriophage ϕX174. XXXVI. Measurement of virus-specific proteins during a normal cycle of infection. Journal of Virology 6:310–319
    [Google Scholar]
  18. PATERSON R., KNIGHT C. A. 1975; Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus. Virology 64:10–22
    [Google Scholar]
  19. PEDEN K. W. C., SYMONS R. H. 1973; Cucumber mcsaic virus contains a functionally divided genome. Virology 53:487–492
    [Google Scholar]
  20. ROBERTS B. E., PATERSON B. M., SPERLING R. 1974; The cell-free synthesis and assembly of viral specific polypeptides into TMV particles. Virology 59:307–313
    [Google Scholar]
  21. SAKAI F., TAKEBE J. 1970; RNA and protein synthesis in protoplasts isolated from tobacco leaves. Biochimica et Biophysica Acta 224:531–540
    [Google Scholar]
  22. SAKAI F., TAKEBE J. 1972; A non-coat protein synthesised in tobacco mesophyll protoplasts infected by tobacco mosaic virus. Molecular and General Genetics 118:93–96
    [Google Scholar]
  23. SAKAI F., TAKEBE I. 1974; Protein synthesis in tobacco protoplasts induced by tobacco mosaic virus infection. Virology 62:426–433
    [Google Scholar]
  24. SÄNGER H. L., KNIGHT C. A. 1963; Action of actinomycin D on RNA synthesis in healthy and virus-infected tobacco leaves. Biochemical and Biophysical Research Communications 13:455–461
    [Google Scholar]
  25. SCHWINGHAMER M. W., SYMONS R. H. 1975; Fractionation of cucumber mosaic virus RNA and its translation in a wheat embryo cell-free system. Virology 63:252–262
    [Google Scholar]
  26. SHAPIRO D., KOS K. A., RUSSELL P. K. 1973; Protein synthesis in Japanese encephalitis virus-infected cells. Virology 56:95–109
    [Google Scholar]
  27. SHIH D. S., KAESBERG P. 1973; Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proceedings of the National Academy of Sciences of the United States of America 70:1799–1803
    [Google Scholar]
  28. SINGER B. 1971; Protein synthesis in virus-infected plants. I. The number and nature of TMV-directed proteins detected on Polyacrylamide gels. Virology 46:247–255
    [Google Scholar]
  29. SINGER B., CONDIT C. 1974; Protein synthesis in virus-infected plants. III. Effects of tobacco mosaic virus mutants on protein synthesis in. Nicotiana tabacum. Virology 57:42–48
    [Google Scholar]
  30. WOOD K. R., COUTTS R. H. A. 1975; Preliminary studies on the RNA components of three strains of cucumber mosaic virus. Physiological Plant Pathology 7:139–145
    [Google Scholar]
  31. ZAITLIN M., HARIHARASUBRAMANIAN V. 1970; Proteins in tobacco mosaic virus-infected tobacco plants. Biochemical and Biophysical Research Communications 39:1031–1036
    [Google Scholar]
  32. ZAITLIN M., HARIHARASUBRAMANIAN V. 1972; A gel electrophoretic analysis of proteins from plants infected with tobacco mosaic and potato spindle tuber viruses. Virology 47:296–305
    [Google Scholar]
  33. ZIEMIECKI A. 1974; Protein synthesis in virus infected cucumber cotyledons. Ph.D. Thesis, University of Birmingham
    [Google Scholar]
  34. ZIEMIECKI A, WOOD K. R. 1975a; Changes in the soluble protein constitution of cucumber cotyledons following infection with two strains of cucumber mosaic virus. Physiological Plant Pathology 7:79–89
    [Google Scholar]
  35. ZIEMIECKI A., WOOD K. R. 1975b; Serological demonstration of virus-specified proteins associated with cucumber mosaic virus infection of cucumber cotyledons. Physiological Plant Pathology 7:171–177
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-3-373
Loading
/content/journal/jgv/10.1099/0022-1317-31-3-373
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error