1887

Abstract

SUMMARY

The rate of incorporation of 5-fluorouracil into complementary strands of replicating RNA of fowl plague virus (FPV) has been studied. The efficiency of incorporation was estimated by determination of the reversion frequency in S-mutants with known types of base transitions in the RNA of the virus particle. It was established that maximum incorporation of 5-fluorouracil into progeny virus particle RNA took place between 2 and 4 h after infection. The maximum incorporation of the mutagen into complementary RNA (plus strands) occurred when the cells were exposed to 5-fluorouracil from 1 to 2 h after infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-3-281
1976-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/3/JV0310030281.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-3-281&mimeType=html&fmt=ahah

References

  1. BALTIMORE D. 1969 Replication of picornaviruses. The Biochemistry of Viruses101–176 Edited by Levy M. B. New York and London: Marcel Dekker;
    [Google Scholar]
  2. BLAIR C. D., ROBINSON W. S. 1968; Replication of Sendai virus. I. Comparison of viral RNA and virus-specific RNA synthesis to Newcastle disease virus. Virology 35:537–549
    [Google Scholar]
  3. COOPER P. D. 1964; The mutation of poliovirus by 5-fluorouracil. Virology 22:186–192
    [Google Scholar]
  4. FLAMAND A., PRINGLE C. 1971; The homologies of spontaneous and induced temperature-sensitive mutants of vesicular stomatitis virus isolated in chick embryo and BHK 21 cells. Journal of General Virology 11:81–85
    [Google Scholar]
  5. FREESE E. 1959; The specific mutagenic effect of base analogues on phage T4. Journal of Molecular Biology 1:87–105
    [Google Scholar]
  6. GERMANOV A. B., PARASIUK N. A. 1972 The possible types of transitions in RNA of S- mutants of FPV. The Molecular Biology of Viruses124–126 Moscow: AMS USSR;
    [Google Scholar]
  7. GERMANOV A.B., SOKOLOV M.I., PARASIUK N. A. 1972; The application of DEAE-cellulose column chromatography to the selection of S+ revertants and determination of reversion frequency in population of S- mutants of fowl plague virus. Journal of General Virology 17:49–54
    [Google Scholar]
  8. GERMANOV A. B., ZAKSTELSKAYA L. Ya., MOLIBOG E. B., EVSTIGNEEVA N. A., PARASIUK N. A., KORNILAEVA G. V. 1974; Neuraminidase mutants of fowl plague virus. Intervirology 2:273–279
    [Google Scholar]
  9. GHARPURE M. A., WRIGHT P. F., CHANOCK R. M. 1969; Temperature-sensitive mutants of respiratory syncytial virus. Journal of Virology 3:414–421
    [Google Scholar]
  10. IKEGAMI N., GOMATOS P. J. 1968; Temperature-sensitive conditional lethal mutants of reovirus. 3. Isolation and characterisation. Virology 36:447–458
    [Google Scholar]
  11. KINGSBURY D. 1970; Replication and function of myxovirus ribonucleic acid. Progress in Medical Virology 12:49–77
    [Google Scholar]
  12. PANIKER C. K. 1968; Serological relationships between the neuraminidases of influenza viruses. Journal of General Virology 2:385–394
    [Google Scholar]
  13. SCHOLTISSEK C., DRZENIEK R., ROTT R. 1969 Myxoviruses. The Biochemistry of Viruses219–258 Edited by Levy M. B. New York and London: Marcel Dekker;
    [Google Scholar]
  14. SCHOLTISSEK C., ROTT R. 1970; Synthesis in vivo of influenza virus plus and minus strand RNA and its inhibition by antibiotics. Virology 40:989–996
    [Google Scholar]
  15. TAN K. B., SAMBROOK J. F., BALLETT A. J. 1969; Semliki forest virus temperature-sensitive mutants. Isolation and characterisation. Virology 38:427–439
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-3-281
Loading
/content/journal/jgv/10.1099/0022-1317-31-3-281
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error