1887

Abstract

SUMMARY

Preparations of broad bean true mosaic virus (BBTMV) contained three centrifugal components: top, middle, and bottom. These had sedimentation coefficients of 59, 95, and 116S. Top component was only found when a reducing agent was used during all stages of purification and subsequent storage. When electrophoresed on acrylamide gels, particles of BBTMV migrated as two, and sometimes three, electrophoretic forms. In new infections a slower migrating form predominated; in older infections a faster migrating form predominated. The relative migration rates of the electrophoretic forms depended on the kind of infected leaf sampled, the environmental conditions during infection, the method of purification, and the period of storage after purification. The changes in mobility of the various forms are ascribed to limited proteolysis, which increased the negative charge of the virus particle by altering the charge of the coat protein. The coat protein is made up of two polypeptides, of mol. wt. 37500 and 20000 to 24500, respectively. The change in charge resulted from a decrease in size, and possibly a change in conformation, of the smaller polypeptide.

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1976-05-01
2021-10-28
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References

  1. AGRAWAL H. O. 1964; Identification of cowpea mosaic viras isolates. Mededelingen Landbouwhogeschool Wageningen 64:1–53
    [Google Scholar]
  2. BANCROFT J. B. 1962; Purification and properties of bean pod mottle virus and associated centrifugal and electrophoretic components. Virology 16:419–427
    [Google Scholar]
  3. CHRAMBACH A., REISFELD R. A., WYCKOFF M., ZACCARI J. 1967; A procedure for rapid and sensitive staining of protein fractionated by Polyacrylamide gel electrophoresis. Analytical Biochemistry 20:150–154
    [Google Scholar]
  4. DAVIS B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  5. DUNKER A. K., RUECKERT R. R. 1969; Observations en molecular weight determinations on Polyacrylamide gel. Journal of Biological Chemistry 244:5074–5080
    [Google Scholar]
  6. GEELEN J. L. M. C, REZELMAN G., VAN KAMMEN A. 1973; The infectivity of the two electrophoretic forms of cowpea mosaic virus. Virology 51:279–286
    [Google Scholar]
  7. GEELEN J. L. M. C, VAN KAMMEN A., VERDUIN B. J. M. 1972; Structure of the capsid of cowpea mosaic virus. The chemical subunit: molecular weight and number of subunits per particle. Virology 49:205–213
    [Google Scholar]
  8. GIBBS A. J., GIUSSANI-BELLI G., SMITH H. G. 1968; Broad-bean stain and true broad-bean mosaic viruses. Annals of Applied Biology 61:99–107
    [Google Scholar]
  9. GILLASPIE A. G., BANCROFT J. B. 1965; The rate of accumulation, specific infectivity, and electrophoretic characteristics of bean pod mottle virus in bean and soybean. Phytopathology 55:906–908
    [Google Scholar]
  10. HARRISON B. D., FINCH J. T., GIBBS A. J., HOLLINGS M., SHEPHERD R. J., VALENTA V., WETTER C. 1971; Sixteen groups of plant viruses. Virology 45:356–363
    [Google Scholar]
  11. HEDRICK J. L., SMITH A. J. 1968; Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Archives of Biochemistry and Biophysics 126:155–164
    [Google Scholar]
  12. NIBLETT C. L., SEMANCIK J. S. 1969; Conversion of the electrophoretic forms of cowpea mosaic virus in vivo and in vitro. Virology 38:685–693
    [Google Scholar]
  13. PAUL H. L. 1963/64; Untersuchungen ueber das Echte Ackerbohnenmosaik-Virus. Phytopathologische Zeitschrift 43:315–319
    [Google Scholar]
  14. SHAPIRO A. L., VINUELA E., MAKEL J. V. JUN. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  15. VAN KAMMEN A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:633–642
    [Google Scholar]
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