1887

Abstract

SUMMARY

A new procedure for the purification of cauliflower mosaic virus (CaMV) which involves the use of Triton X-100 and urea results in higher and more reliable yields. CaMV particles have a of 208S, a of 0µ75 × 10 cm/s, contain 17% nucleic acid and have a mol. wt. of 22.8 × 10. Virus precipitated by Mg, low pH or ethanol at moderate salt concentrations, or by polyethylene glycol, is not easily resuspended.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-1-93
1976-04-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/1/JV0310010093.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-1-93&mimeType=html&fmt=ahah

References

  1. BRUNT A. 1971; Some hosts and properties of dahlia mosaic virus. Annals of Applied Biology 67:357–368
    [Google Scholar]
  2. BRUNT A. A., BARTON R. J., TREMAINE J. H., STACE-SMITH R. 1975; The composition of cauliflower mosaic virus protein. Journal of General Virology 27:101–106
    [Google Scholar]
  3. BURTON K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colori-metric estimation of deoxyribonucleic acid. Biochemical Journal 6a:315–323
    [Google Scholar]
  4. COHEN G., EISENBERG H. 1968; Deoxyribonucleate solutions: sedimentation in a density gradient, partial specific volumes, refractive index measurements and preferential interactions. Biopolymers 6:1077–1100
    [Google Scholar]
  5. DUNN D. B., HITCHBORN J. H. 1965; The use of bentonite in the purification of plant viruses. Virology 35:171–192
    [Google Scholar]
  6. ENGLANDER S. W., EPSTEIN H. T. 1957; Optical methods for measuring nucleoprotein and nucleic acid concentrations. Archives of Biochemistry and Biophysics 68:144–149
    [Google Scholar]
  7. FABERGé A. C., OLIVER R. M. 1974; Methylamine tungstate, a new negative stain. Journal de Microscopie 20:241–246
    [Google Scholar]
  8. FARRELL J. A., HARVEY J. D., BELLAMY A. R. 1974; Biophysical studies of reovirus type 3.1. The molecular weights of reovirus and reovirus cores. Virology 62:145–153
    [Google Scholar]
  9. FUJISAWA I., RUBIO-HUERTOS M., MATSUI C., YAMAGUCHI A. 1967; Intracellular appearance of cauliflower mosaic virus particles. Phytopathology 57:1130–1132
    [Google Scholar]
  10. GÖMEC B. 1973; Properties and biochemical characterization of dahlia mosaic virus. Ph.D. Thesis, University of California, Davis 44
    [Google Scholar]
  11. HARVEY J. D. 1973; Diffusion coefficients and hydrodynamic radii of three spherical RNA viruses by laser light scattering. Virology 56:365–368
    [Google Scholar]
  12. HARVEY J. D., FARRELL J. A., BELLAMY A. R. 1974; Biophysical studies of reovirus type 3. II. Properties of the hydrated particle. Virology 62:154–160
    [Google Scholar]
  13. HILLS G. J., CAMPBELL R. N. 1968; Morphology of broccoli necrotic yellows virus. Journal of Ultrastructural Research 24:134–144
    [Google Scholar]
  14. HULL R., HILLS G. J., MARKHAM R. 1969; Studies on alfalfa mosaic virus. II. The structure of the virus components. Virology 37:416–428
    [Google Scholar]
  15. ITOH T., MATSUI C., HIRAI T. 1969; Conformational changes in cauliflower mosaic virus. Virology 39:367–372
    [Google Scholar]
  16. KELLY D. C, COOPER V., WALKEY D. O. A. 1974; Cauliflower mosaic virus structural proteins. Microbios 10:239–245
    [Google Scholar]
  17. LUNG M. C. Y., PIRONE T. P. 1973; Studies on the reason for differential transmissibility of cauliflower mosaic virus isolates by aphids. Phytopathology 63:910–914
    [Google Scholar]
  18. NAKAMURA G. R. 1952; Microdetermination of phosphorus. Analytical Chemistry 24:1372
    [Google Scholar]
  19. PIRONE T. P., POUND G. S., SHEPHERD R. J. 1960; Purification and properties of cauliflower mosaic virus. Nature, London 186:656–657
    [Google Scholar]
  20. PIRONE T. P., POUND G. S., SHEPHERD R. J. 1961; Properties and serology of purified cauliflower mosaic virus. Phytopathology 51:541–546
    [Google Scholar]
  21. PUSEY P. N., KOPPEL D. E., SCHAEFER R. D., CAMERINI-OTERO R. D., KOENIG S. H. 1974; Intensity fluctuation spectroscopy of laser light scattered by solutions of spherical viruses: R17, , BSV, PM2 and T7. I. Light scattering technique. Biochemistry 13:952–959
    [Google Scholar]
  22. PUSEY P. N., SCHAEFER D. W., KOPPEL D. E., CAMERINI-OTERO R. D., FRANKLIN R. M. 1972; A Study of the diffusion properties of R17 virus by time dependent light scattering. Journal de Physique 33:C1163–168
    [Google Scholar]
  23. RUSSELL G. J., FOLLETT E. A. C, SUBAK-SHARPE J. H., HARRISON B. D. 1971; The double-stranded DNA of cauliflower mosaic virus. Journal of General Virology 11:129–138
    [Google Scholar]
  24. SHEPHERD R. J. 1970; Cauliflower mosaic virus. CMI/AAB Description of Plant Viruses No. 24
    [Google Scholar]
  25. SHEPHERD R. J., BRUENING G. E., WAKEMAN R. J. 1970; Double-stranded DNA from cauliflower mosaic virus. Virology 41:339–347
    [Google Scholar]
  26. SHEPHERD R. J., WAKEMAN R. J. 1971; Observation on the size and morphology of cauliflower mosaic virus deoxyribonucleic acid. Phytopathology 61:188–193
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-1-93
Loading
/content/journal/jgv/10.1099/0022-1317-31-1-93
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error