1887

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Volume 31, Issue 1

Effects of the Phage P1 Restriction System on Coliphage φW: Degradation and Complex Formation of Phage φW DNA Free

Abstract

SUMMARY

Growth of phages φW and T7 was restricted in lysogenic for phage P1. Only a fraction of the infected cells gave burst of phages. Cells permitting phage growth gave normal burst size. Host strains carrying P1 mutants with defective endonuclease gave no restriction of phages T7 and φ3, the latter a host-range mutant of φW. Degradation but not modification of parental phage DNA could be demonstrated. Although no DNA, RNA or protein was synthesized in φW infected P1 lysogenic cells, the parental phage DNA was found in increasingly larger complexes during the course of infection. At early times after infection, parental phage DNA was found to sediment about twice as fast as mature phage DNA. At later times during the infection the parental phage DNA was recovered as a very rapidly sedimenting material. Such material was also found in alkaline sucrose gradient centrifugation after treatment of the cell extract with sodium dodecyl sulphate, pronase digestion and phenol extractions.

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  • Published Online:
© Society for General Microbiology 1976
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1976-04-01
2023-06-06
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References

  1. ADAMS M. H. 1959 Bacteriophages New York: Interscience Publishers;
     [Google Scholar]
  2. ANDERSON E. H. 1946; Growth requirements of virus-resistant mutants of Escherichia colis. Proceedings 0 the National Academy of Sciences of the United States of America 32:120–128
     [Google Scholar]
  3. ARBER W. 1974; DNA modification and restriction. Progress in Nucleic Acid Research and Molecular Biology 14:1–37
     [Google Scholar]
  4. BEIER H., HAUSMANN R. 1973; Genetic map of bacteriophage T3. Journal of Virology 12:417–419
     [Google Scholar]
  5. BERTANI G. 1951; Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. Journal of Bacteriology 62:293–300
     [Google Scholar]
  6. BOMAN H. G., MONNER D. 1975; Characterization of lipopolysaccharides from Escherichia coli k-12 mutants. Journal of Bacteriology 121:455–464
     [Google Scholar]
  7. BRITTON J. R., HASELKORN R. 1975; Permeability lesions in male Escherichia coli infected with bacteriophage T7. Proceedings of the National Academy of Sciences of the United States of America 72:2222–2226
     [Google Scholar]
  8. BRUNOVSKIS I., BURNS R. O. 1973; Growth of coliphage T7 in Salmonella typhimurium. Journal of Virology 11:621–629
     [Google Scholar]
  9. CENTER M. S. 1972; Replicative intermediates of bacteriophages T7 deoxyribonucleic acid. Journal of Virology 10:115–123
     [Google Scholar]
  10. CUZIN M. F. 1965; Un bacteriophage spécifique du type sexuel F-d’Escherichia coli K12. Compte rendue de VAcademie des sciences, Paris 260:6482–6485
     [Google Scholar]
  11. ESKIN B., LAUTENBERGER J. A., LINN S. 1973; Host-controlled modification and restriction of bacteriophage T7 by Escherichia coli b. Journal of Virology 11:1020–1023
     [Google Scholar]
  12. ESKRIDGE R. W., WEINFELD H., PAIGEN K. 1967; Susceptibility of different coliphage genomes to host-controlled variation. Journal of Bacteriology 93:835–844
     [Google Scholar]
  13. GEFTER M., HAUSMANN R., GOLD M., HURWITZ J. 1966; The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. X. Bacteriophage T3-induced S-adenosylmethionine cleavage. Journal of Biological Chemistry 241:1995–2006
     [Google Scholar]
  14. HATTMAN S. 1964; The control of host-induced modification by phage P1. Virology 23:270–271
     [Google Scholar]
  15. HAUSMANN R. 1973 Host dependent expression of bacteriophages T3 and T7. Molecular Cytogenetics263–276 Edited by Hamkalo B. A., Papaconstantinou J. New York: Plenum Publ. Co;
     [Google Scholar]
  16. HERRLICH P., SCHWEIGER M. 1970; T3 and T7 bacteriophage deoxyribonucleic acid-directed enzyme synthesis in vitro. Journal of Virology 6:750–753
     [Google Scholar]
  17. HYMAN R. W., BRUNOVSKIS I., SUMMERS W. C. 1974; A biochemical comparison of the related bacteriophages T7, ϕI, (ϕII, W3I, H and T3. Virology 57:189–206
     [Google Scholar]
  18. KELLY T. JUN., THOMAS C. A. JUIST 1969; An intermediate in the replication of bacteriophage T7 DNA molecules. Journal of Molecular Biology 44:459–475
     [Google Scholar]
  19. LEDERBERG S. 1957; Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria. Virology 3:496–513
     [Google Scholar]
  20. MäKELä O., MÄKELÄ P. H., SOIKKELI S. 1964; Sex-specificity of the bacteriophage T7. Annales medicine experimentalis et biologiae Fenniae 42:188–195
     [Google Scholar]
  21. MILLER J. H. 1972; Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
     [Google Scholar]
  22. MONNER D. A., BOMAN H. G. 1970; Female strains of Escherichia coli k-12 as selective hosts for the isolation of female specific mutants of phage ϕII. Biochemical Biophysical Research Communications 39:1017–1020
     [Google Scholar]
  23. MONNER D. A., BOMAN H. G. 1974; Host-dependent properties of coliphage ϕW and its female specific host-range mutants, ϕ3 and ϕ4. Journal of General Virology 24:67–75
     [Google Scholar]
  24. MONNER D. A., JONSSON S., BOMAN H. O. 1971; Ampicillin-resistant mutants of Escherichia coli k-12 with lipopolysaccharide alterations affecting mating ability and susceptibility to sex-specific bacteriophages. Journal of Bacteriology 107:420–432
     [Google Scholar]
  25. MORRISON T. G., MALAMY M. H. 1971; T7 translational control mechanism and their inhibition by F-factors. Nature New Biology 231:37–41
     [Google Scholar]
  26. NEIDHARDT F. C, BLOCH P. L., SMITH D. F. 1974; Culture medium for Enterobacteria. Journal of Bacteriology 119:736–747
     [Google Scholar]
  27. PACUMBABA R. P., CENTER M. S. 1973; Association of replicating bacteriophage T7 DNA with bacterial membranes. Journal of Virology 12:855–861
     [Google Scholar]
  28. REEVE E. C. R., DOHERTY P. 1968; Linkage relationships of two genes causing partial resistance to chloramphenicol in Escherichia coli. Journal of Bacteriology 96:1450–1451
     [Google Scholar]
  29. SCHELL J., GLOVER S. W., STACEY K. A., BRODA P. M. A., SYMONDS N. 1963; The restriction of phage T3 by certain strains of Escherichia coli. Genetical Research, Cambridge 4:483–484
     [Google Scholar]
  30. SIGNER E. R., BECKWITH J. R. 1966; Transposition of the Lac region of Escherichia coli III. The mechanism of attachment of bacteriophage ϕ80 to the bacterial chromosome. Journal of Molecular Biology 22:33–51
     [Google Scholar]
  31. SMITH O. H., WILCOX K. W. 1970; A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. Journal of Molecular Biology 51:379–391
     [Google Scholar]
  32. STUDIER F. W. 1972; Bacteriophage T7. Science, New York 176:367–376
     [Google Scholar]
  33. VOGEL H. J., BONNER D. M. 1956; Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
     [Google Scholar]
  34. WILLIAMS L., MEYNELL G. G. 1971; Female-specific phages and F-minus strains of Escherichia coli k-12. Molecular and General Genetics 113:222–227
     [Google Scholar]
  35. WOLLMAN E. 1947; Relations entre le pouvoir de synthétiser la proline et la resistance au bacteriophage chez des mutants à’Escherichia coli. Annals of the Institute Pasteur, Paris 73:348–363
     [Google Scholar]
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Effects of the Phage P1 Restriction System on Coliphage φW: Degradation and Complex Formation of Phage φW DNA
J. Gen. Virol. 31, 9 (1976); https://doi.org/10.1099/0022-1317-31-1-9
/content/journal/jgv/10.1099/0022-1317-31-1-9
/content/journal/jgv/10.1099/0022-1317-31-1-9
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