1887

Abstract

SUMMARY

Eleven temperature-sensitive mutants of herpes simplex virus type 2 strain 52 were examined for ability to induce DNA polymerase activity in BHK 21/C13 cells. All mutants induced DNA polymerase at a permissive temperature, (31 °C) and all DNA-positive mutants at a non-permissive temperature (38 °C). Three DNA-negative mutants induced no DNA polymerase ( 6, 9) or very little DNA polymerase ( 11), at a non-permissive temperature, while 1, also DNA negative, induced a little more DNA polymerase than wild-type, often at both temperatures. The DNA polymerase induced by 6 at 31 °C was temperature-sensitive , but only slightly so . These results were confirmed immunologically and suggest that HSV-2 codes for at least part of a DNA polymerase activity, necessary for infection, and that full expression of this enzyme involves at least three viral genes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-1-65
1976-04-01
2024-11-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/1/JV0310010065.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-1-65&mimeType=html&fmt=ahah

References

  1. ARON G. M., PURIFOY D. J. M., SCHAFFER P. A. 1975; DNA synthesis and DNA polymerase activity of herpes simplex virus type 1 temperature-sensitive mutants. in the press
  2. ESPARZA J., PURIFOY D. J. M., SCHAFFER P. A., BENYESH-MELNICK M. 1974; Isolation, complementation and preliminary characterisation of temperature-sensitive mutants of herpes simplex virus type 2. Virology 57:554–565
    [Google Scholar]
  3. HALLIBURTON I. W., TIMBURY M. C. 1973; Characteristics of temperature-sensitive mutants of herpes simplex virus type 2. Growth and DNA synthesis. Virology 54:60–68
    [Google Scholar]
  4. HAY J., MOSS H., HALLIBURTON I. W. 1971a; Indication of deoxyribonucleic acid polymerase and deoxy-ribonuclease activities in cells infected with herpes simplex virus type 2. Biochemical Journal 124:64
    [Google Scholar]
  5. HAY J., PERERA P. A. J., MORRISON J. M., GENTRY G. A., SUBAK-SHARPE H. 1971b HerpeSVfrUS-Specified proteins. Strategy of the Viral Genome355–376 Edited by Wolstenholme G., O’Connor M. Ciba Foundation Symposium;
    [Google Scholar]
  6. HOWARD D. K., HAY J., MELVIN W. T., DURHAM J. 1974; Changes in DNA and RNA synthesis and associated enzyme activities after the stimulation of serum-depleted BHK 21/C13 cells by the addition of serum. Experimental Cell Research 86:31–42
    [Google Scholar]
  7. JAMIESON A. T., SUBAK-SHARPE J. H. 1974; Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity. Journal of General Virology 24:481–492
    [Google Scholar]
  8. KEIR H. M., HAY J., MORRISON J. M., SUBAK-SHARPE J. H. 1966; Altered properties of DNA nucleotidyl transferase after infection of mammalian cells with herpes simplex virus. Nature, London 210:369–371
    [Google Scholar]
  9. PRINGLE C. R., HOWARD D. K., HAY J. 1973; Temperature-sensitive mutants of Pseudorabies virus with differential effects on viral and host DNA synthesis. Virology 55:495–505
    [Google Scholar]
  10. PURIFOY D. J. M., BENYESH-MELNICK M. 1974; DNA polymerase induction by DNA-negative temperature-sensitive (ts) mutants of herpes simplex virus type 2 (HSV-2). Abstracts of the Annual Meeting of the Society for Microbiology234
    [Google Scholar]
  11. PURIFOY D. J. M., BENYESH-MELNICK M. 1975; DNA polymerase induction by DNA-negative temperature-sensitive mutants of herpes simplex virus type 2. in the press
  12. SUBAK-SHARPE J. H., BROWN S. M., RITCHIE D. A., TIMBURY M. C, MACNAB J. C. M., MARSDEN H. S., HAY J. 1975; Genetic and biochemical studies with herpesvirus. Cold Spring Harbor Symposium on Quantitative Biology 39:717–730
    [Google Scholar]
  13. THOULESS M. E., SKINNER G. R. B. 1971; Differences in the properties of thymidine kinase produced in cells infected with type 1 and type 2 herpes virus. Journal of General Virology 12:195–197
    [Google Scholar]
  14. TIMBURY M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–375
    [Google Scholar]
  15. TIMBURY M. C., HAY J. 1975; Genetic and physiological studies with type 2 herpes simplex virus ts mutants. Oncogenesis and Herpesviruses in the press
    [Google Scholar]
  16. WEISSBACH A., HONG S-C. L., AUCKER J., MULLER R. 1973; Characterisation of herpes simplex virus-induced deoxyribonucleic acid polymerase. Journal of Biological Chemistry 248:6270–6277
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-31-1-65
Loading
/content/journal/jgv/10.1099/0022-1317-31-1-65
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error