1887

Abstract

SUMMARY

Three mutants of herpes simplex virus (HSV) have been isolated which form plaques in the presence of 100 µg/ml phosphonacetic acid (PPA). All three mutants (3 from HSV-1 strain 17 , from HSV-1 strain 17 , and 19 from HSV-2) induce viral DNA synthesis and viral DNA polymerase activity, and these are much less sensitive to PPA than the wild-type virus. The results support the hypothesis that PPA interacts directly with the viral DNA polymerase protein, at least part of which is virus coded.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-1-145
1976-04-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/1/JV0310010145.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-1-145&mimeType=html&fmt=ahah

References

  1. ARON G. M., PURIFOY D. J. M., SCHAFFER P. 1975; DNA synthesis and DNA polymerase activities of herpes simplex virus type 1 ts mutants. Journal of Virology 16:498–511
    [Google Scholar]
  2. BERKOWITZ D. M., KAKEFUDA T., SPORN M. B. 1969; A simple and rapid method for the isolation of enzymatically active HeLa cell nuclei. Journal of Cell Biology 42:851–855
    [Google Scholar]
  3. BOLDEN A., AUCKER J., WEISSBACH A. 1975; Synthesis of herpes vaccinia and adenovirus DNA in isolated HeLa cell nuclei. I. Effect of viral specific antisera and phosphonoacetic acid. Journal of Virology in the press
    [Google Scholar]
  4. CROMBIE I. K. 1975; Genetic and biochemical studies with herpes simplex virus type 1. Ph.D. Thesis, University of Glasgow
    [Google Scholar]
  5. HAY J., MOSS H. M., JAMIESON A. T., TIMBURY M. C. 1976; Herpes virus proteins: DNA polymerase and pyrimidine deoxynucleoside kinase activities in temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 31:65–73
    [Google Scholar]
  6. KEIR H. M., HAY I., MORRISON J. M., SUBAK-SHARPE J. H. 1966; Altered properties of deoxyribonucleic acid nucleotidyl transferase after infection of mammalian cells with herpes simplex virus. Nature, London 210:369–371
    [Google Scholar]
  7. MAO J. C.-H., ROBISHAW E. E., OVERBY L. R. 1975; Inhibition of DNA polymerase activity from herpes simplex infected W138 cells by phosphonoacetic acid. Journal of Virology 15:1281–1283
    [Google Scholar]
  8. OVERBY L. R., ROBISHAW E. E., SCHLEICHER J. B., RUTER A., SHIPKOWITZ N. L., MAO J. C.-H. 1974; Phosphonoacetic acid: inhibitor of herpes simplex virus. Antimicrobial Agents and Chemotherapy 6:360–365
    [Google Scholar]
  9. PURIFOY D. J. M., BENYESH-MELNICK M. 1976; DNA polymerase induction by DNA-negative temperature-sensitive mutants of herpes simplex virus type 2. Submitted for publication
  10. RUSSELL W. C. 1962; A sensitive and precise plaque assay for herpes simplex virus. Nature, London 19s:1028–1029
    [Google Scholar]
  11. SHIPKOWITZ N. L., BOWER R. R., APPELL R. N., NORDEEN C. W., OVERBY L. R., RODERICK W. R., SCHLEICHER J. B., VON ESCH A. M. 1973; Suppression of herpes simplex virus infection by phosphonoacetic acid. Applied Microbiology 27:264–267
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-1-145
Loading
/content/journal/jgv/10.1099/0022-1317-31-1-145
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error