1887

Abstract

SUMMARY

The human papovavirus, BK, appeared weakly oncogenic in newborn hamsters and was able to induce transformation of rat kidney cells. The infectivity of BK virus DNA was determined by employing the DEAE-dextran method. In human embryonic cells the infectivity was approx. 10 p.f.u./g of DNA. The transforming ability of BK virus in primary rat kidney cells was measured by employing the calcium method and appeared to vary from 1 to 10 foci per g of DNA.

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/content/journal/jgv/10.1099/0022-1317-30-3-371
1976-03-01
2022-01-20
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References

  1. Aay C., Borst P. 1972; The gel electrophoresis of DNA. Biochimica et Biophysica Acta 269:192–200
    [Google Scholar]
  2. Abrahams P. J., Van Der EB A. J. 1975; In vitro transformation of rat and mouse cells by DNA from simian virus 40. Journal of Virology 16:206–209
    [Google Scholar]
  3. Gardner S. D., Field A. M., Coleman D. V., Hulme B. 1971; New human papovavirus (BK) isolated from urine after renal transplantation. The Lancet 1:1253–1257
    [Google Scholar]
  4. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  5. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  6. Mccutchan J. H., Pagano J. S. 1968; Enhancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl dextran. Journal of the National Cancer Institute 41:351–357
    [Google Scholar]
  7. Mayor E. O., Di Mayorca G. 1973; Malignant transformation of BHK 21 Clone 13 cells by BK virus – a human papovavirus. Proceedings of the National Academy of Sciences of the United States of America 70:3210–3212
    [Google Scholar]
  8. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA; the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 57:1514–1521
    [Google Scholar]
  9. Shah K. V., Daniel R. W., Strandber J. D. 1975; Sarcoma in a hamster inoculated with BK virus, a human papovavirus. Journal of the National Cancer Institute 54:945–947
    [Google Scholar]
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