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Abstract
A cytoplasmic polyhedrosis virus (CPV) isolated from larvae of Arctia caja contained at least 12 RNA segments when these were fractionated by poly-acrylamide gel electrophoresis. When the virus RNA was compared with RNA isolated from Nymphalis io CPV and from Spodoptera exempta CPV, it appeared that A. caja CPV could be interpreted as a mixture of these two viruses. The structural polypeptides of virus particles of A. caja CPV also resembled a mixture of proteins derived from the other two virus types, but two classes of particles were not separated by size or density measurements. Virus particles of S. exempta CPV and N. io CPV were unrelated serologically using antisera that did not react with double-stranded RNA. Particles of A. caja CPV were resolved into two fractions using the antiserum specific for S. exempta CPV; particles containing RNA segments characteristic of S. exempta CPV and particles containing RNA segments characteristic of N. io CPV. This confirmed that the A. caja CPV isolate was a naturally-occurring mixture of two virus types.
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