1887

Abstract

SUMMARY

A cytoplasmic polyhedrosis virus (CPV) isolated from larvae of contained at least 12 RNA segments when these were fractionated by poly-acrylamide gel electrophoresis. When the virus RNA was compared with RNA isolated from CPV and from CPV, it appeared that CPV could be interpreted as a mixture of these two viruses. The structural polypeptides of virus particles of CPV also resembled a mixture of proteins derived from the other two virus types, but two classes of particles were not separated by size or density measurements. Virus particles of CPV and CPV were unrelated serologically using antisera that did not react with double-stranded RNA. Particles of CPV were resolved into two fractions using the antiserum specific for CPV; particles containing RNA segments characteristic of CPV and particles containing RNA segments characteristic of CPV. This confirmed that the CPV isolate was a naturally-occurring mixture of two virus types.

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/content/journal/jgv/10.1099/0022-1317-30-3-357
1976-03-01
2021-10-19
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References

  1. Aruga H., Tanada Y. 1971 In The Cytoplasmic Polyhedrosis Virus of the Silkworm Tokyo: University of Tokyo Press;
    [Google Scholar]
  2. Cunningham J. C., Longworth J. F. 1968; The identification of some cytoplasmic polyhedrosis viruses. Journal of Invertebrate Pathology n:196–202
    [Google Scholar]
  3. Fujii-Kawata I., Miura K., Fuke M. 1970; Segments of genome of viruses containing double-stranded ribonucleic acids. Journal of Molecular Biology 51:247–253
    [Google Scholar]
  4. Hayashi Y., KryWienczyk J. 1972; Electrophoretic fractionation of cytoplasmic polyhedrosis virus genome. Journal of Invertebrate Pathology 19:160–162
    [Google Scholar]
  5. Ikegami M., Francki R. I. B. 1973; Presence of antibodies to double-stranded RNA in sera of rabbits immunized with rice dwarf and maize rough dwarf viruses. Virology 56:404–406
    [Google Scholar]
  6. Kalmakoff J., Payne C. C. 1973; A simple method for the separation of single-stranded and doublestranded RNA on hydroxyapatite. Analytical Biochemistry 55:26–33
    [Google Scholar]
  7. Lewandowski L. J., Traynor B. L. 1972; Comparison of the structure and polypeptide composition of three double-stranded ribonucleic acid-containing viruses (diplornaviruses): cytoplasmic polyhedrosis virus, wound tumor virus, and reovirus. Journal of Virology 10:1053–1070
    [Google Scholar]
  8. Payne C. C. 1974; The isolation and characterization of a virus from Oryctes rhinoceros. Journal of General Virology 25:105–116
    [Google Scholar]
  9. Payne C. C., Kalmakoff J. 1975a; Biochemical properties of polyhedra and virus particles of the cytoplasmic polyhedrosis virus of Bombyx mori. Intervirology 4:354–364
    [Google Scholar]
  10. Payne C. C., Kalmakoff J. 1975b; A radioimmunoassay for the cytoplasmic polyhedrosis virus from Bombyx mori. Intervirology 4:365–368
    [Google Scholar]
  11. Payne C. C., Tinsley X. W. 1974; The structural proteins and RNA components of a cytoplasmic polyhedrosis virus from Nymphalis io (Lepidoptera: Nymphalidae). Journal of General Virology 25:291–302
    [Google Scholar]
  12. Smith K. M., Wyckoff R. W. G. 1950; Structure within polyhedra associated with insect virus diseases. Nature, London 166:861–862
    [Google Scholar]
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