A cytoplasmic polyhedrosis virus (CPV) isolated from larvae of contained at least 12 RNA segments when these were fractionated by poly-acrylamide gel electrophoresis. When the virus RNA was compared with RNA isolated from CPV and from CPV, it appeared that CPV could be interpreted as a mixture of these two viruses. The structural polypeptides of virus particles of CPV also resembled a mixture of proteins derived from the other two virus types, but two classes of particles were not separated by size or density measurements. Virus particles of CPV and CPV were unrelated serologically using antisera that did not react with double-stranded RNA. Particles of CPV were resolved into two fractions using the antiserum specific for CPV; particles containing RNA segments characteristic of CPV and particles containing RNA segments characteristic of CPV. This confirmed that the CPV isolate was a naturally-occurring mixture of two virus types.


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