1887

Abstract

SUMMARY

An enterovirus (EVU-16) previously isolated from children with acute infectious lymphocytosis has been further characterized. The EVU-16 virus sediments as a 135S particle in sucrose gradients, has a density of 1.335 g/ml in CsCl, contains 4 polypeptides and has a single stranded RNA genome sedimenting at 35S. These structural features as well as the presence of a virus-related particle, the procapsid, are similar to those of other enteroviruses. However, the largest polypeptide of EVU-16 is 49 000 daltons, which is considerably larger than the corresponding polypeptide from poliovirus; the sizes of the other three viral polypeptides were similar in both viruses. Attempts to induce lymphocytosis by the inoculation of EVU-16 into various animals, including immunologically aberrant ‘nude’ mice, were unsuccessful.

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1976-03-01
2024-12-09
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References

  1. Baltimore D. 1968; Structure of the poliovirus replicative intermediate RNA. Journal of Molecular Biology 32:359–368
    [Google Scholar]
  2. Baltimore D., Girard M., Darnell J. E. 1966; Aspects of the synthesis of poliovirus RNA and the formation of virus particles. Virology 29:179–189
    [Google Scholar]
  3. Best M., Evans B., Bishop J. M. 1972; Double-stranded replicative form of poliovirus RNA: phenotype of heterozygous molecules. Virology 47:592–603
    [Google Scholar]
  4. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  5. Borun T. W., Scharff M. D., Robbins E. 1967; Preparation of mammalian polyribosomes with the detergent Nonidet P–40. Biochimica et Biophysica Acta 149:302–304
    [Google Scholar]
  6. Butterworth B. E. 1973; A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus-l A, and poliovirus. Virology 56:439–453
    [Google Scholar]
  7. Fernandez-Tomas C. B., Baltimore D. 1973; Morphogenesis of poliovirus. II. Demonstration of a new intermediate, the provirion. Journal of Virology 12:1122–1130
    [Google Scholar]
  8. Hecht T. T., Summers D. F. 1970; The effect of phleomycin on poliovirus RNA replication. Virology 40:441–447
    [Google Scholar]
  9. Horwitz M. S. 1971; Intermediates in the synthesis of type 2 adenovirus deoxyribonucleic acid. Journal of Virology 8:675–683
    [Google Scholar]
  10. Horwitz M. S., Moore G. T. 1968; Acute infectious lymphocytosis. An etiologic and epidemiologic study of an outbreak. New England Journal of Medicine 279:399–404
    [Google Scholar]
  11. Ifft J. B., Voet D. H., Vinograd J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. Journal of Physical Chemistry 65:1138–1145
    [Google Scholar]
  12. Jacobson M. F., Asso J., Baltimore D. 1970; Further evidence on the formation of poliovirus proteins. Journal of Molecular Biology 49:657–669
    [Google Scholar]
  13. Jacobson M. F., Baltimore D. 1968; Morphogenesis of poliovirus. I. Association of the viral RNA with coat rotein. Journal of Molecular Biology 33:369–378
    [Google Scholar]
  14. Maizel J. V. Jun 1971; Polyacrylamide gel electrophoresis of viral proteins. In Methods in Virology vol 5 Chapter 32, pp 180–246 Edited by Maramorosch, Koprowski H. New York: Academic Press;
    [Google Scholar]
  15. Maizel J. V., Phillips B. A., Summers D. F. 1967; Composition of artificially produced and naturally occurring empty capsids of poliovirus type I. Virology 32:692–699
    [Google Scholar]
  16. Maizel J. V., Summers D. F. 1968; Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells. Virology 36:48–54
    [Google Scholar]
  17. Pantelouris E. M. 1968; Absence of thymus in a mouse mutant. Nature, London 217:370–371
    [Google Scholar]
  18. Penman S., Becker V., Darnell J. E. 1964; A cytoplasmic structure involved in the synthesis and assembly of poliovirus components. Journal of Molecular Biology 8:541–555
    [Google Scholar]
  19. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  20. Summers D. F., Maizel J. V., Darnell J. E. 1965; Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 54:505–513
    [Google Scholar]
  21. Williams J. F. 1970; Enhancement of adenovirus plaque formation on HeLa cells by magnesium chloride. Journal of General Virology 9:251–255
    [Google Scholar]
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