1887

Abstract

SUMMARY

In the presence of poly--ornithine at 1 g/ml, the uptake of [H]-labelled particles of tobacco rattle virus (TRV) by tobacco mesophyll protoplasts during inoculation depended on the virus and protoplast concentrations in the inoculation mixture. Uptake decreased 15- to 30-fold in the absence of poly--ornithine. Substituting phosphate for citrate buffer in the inoculum greatly increased infection but had little effect on virus uptake. For infections detected by staining with fluorescent antibody to virus particles, the ID occurred at an uptake of 30 long TRV particles per protoplast using phosphate buffer, and 250 using citrate. Evidence was obtained that the phosphate-induced enhancement of infection is mediated through an effect on interaction of virus and poly--ornithine during pre-inoculation incubation. Batches of protoplasts differing in susceptibility to infection did not differ similarly in uptake of inoculum virus.

Electron microscopy of sections of freshly inoculated protoplasts revealed single TRV particles associated end-on with normal-looking plasmalemma, and aggregates that contained densely stained material plus TRV particles, associated with breaks in the plasmalemma, and adjacent to areas of intracytoplasmic vesiculation. Some aggregates entered the cytoplasm and some TRV particles entered the vesicles. The aggregates were on average smaller after inoculation with phosphate-containing than with citrate-containing inocula. It is suggested that mini-aggregates of the kind produced during incubation with phosphate play an important role in infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-3-287
1976-03-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/3/JV0300030287.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-3-287&mimeType=html&fmt=ahah

References

  1. Btjrgess J., Motoyoshi F., Fleming E. N. 1973a; The mechanism of infection of plant protoplasts by viruses. Planta (Berlin) 112:323–332
    [Google Scholar]
  2. Burgess J., Motoyoshi F., Fleming E. N. 1973b; Effect of poly-l-ornithine on isolated tobacco mesophyll protoplasts: evidence against stimulated pinocytosis. Planta (Berlin) 111:199–208
    [Google Scholar]
  3. Frost R. R., Harrison B. D., Woods R. D. 1967; Apparent symbiotic interaction between particles of tobacco rattle virus. Journal of General Virology 1:57–70
    [Google Scholar]
  4. Harrison B. D. 1970; Tobacco rattle virus. C.M.I./A.A.B. Descriptions of Plant Viruses No. 12 4 pp
    [Google Scholar]
  5. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. Journal of General Microbiology 21:569–581
    [Google Scholar]
  6. Hibi T., Yora K. 1972; Electron microscopy of tobacco mosaic virus infection in tobacco mesophyll protoplasts. Annals of the Phytopathological Society of Japan 38:350–356
    [Google Scholar]
  7. Kubo S., Harrison B. D., Barker H. 1975b; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
    [Google Scholar]
  8. Kubo S., Harrison B. D., Robinson D. J. 1974; Effect of phosphate on the infection of tobacco protoplasts by tobacco rattle virus. Intervirology 3:382–387
    [Google Scholar]
  9. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975a; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  10. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973a; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  11. Motoyoshi F., Bancroft J. B., Watts J. W. 1973b; A direct estimate of the number of cowpea chlorotic mottle virus particles absorbed by tobacco protoplasts that become infected. Journal of General Virology 21:159–161
    [Google Scholar]
  12. Otsuki Y., Takebe I., Honda Y., Matsui C. 1972; Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology 49:188–194
    [Google Scholar]
  13. Roberts I. M. 1970; Reduction of compression artifacts in ultra-thin sections by the application of heat. Journal of Microscopy 92:57–61
    [Google Scholar]
  14. Roberts I. M. 1975; Tungsten coating -a method of improving glass microtome knives for cutting ultrathin sections. Journal of Microscopy 103:113–119
    [Google Scholar]
  15. Takebe I., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences of the United States of America 64:843–848
    [Google Scholar]
  16. Wyatt S. D., Shaw J. G. 1975; Retention and dissociation of tobacco mosaic virus by tobacco protoplasts. Virology 63:459–465
    [Google Scholar]
  17. Zhuravlev Y. N., Pisetskaya N. F., Shumilova L. A., Musorok T. I., Reifman V. G. 1975; Attachment of labeled TMV to tobacco mesophyll protoplasts. Virology 64:43–48
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-3-287
Loading
/content/journal/jgv/10.1099/0022-1317-30-3-287
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error