%0 Journal Article %A Warocquier, Rita %A Boulanger, P. A. %T Adenovirus Protein Maturation at 42 °C %D 1976 %J Journal of General Virology, %V 30 %N 2 %P 233-242 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-30-2-233 %I Microbiology Society, %X SUMMARY Incubation of adenovirus type 2 infected cells at 42 °C resulted in an inhibition of assembly of virus particles although all the major viral structural polypeptides and virus-induced cellular polypeptides so far identified were detected by electrophoretic analysis. Selective high salt-acid-urea extraction of low mol. wt. polypeptides revealed the absence of protein VII at 42 °C whereas precursor polypeptide P-VII and core protein V were found. Pulse-chase and temperature shift experiments indicated that cleavage of P-VII into VII was a reversible thermosensitive process, requiring de novo protein synthesis after shift-down to 37 °C. Virus particles assembled at 37 °C after transfer from 42 to 37 °C contained both viral DNA and polypeptides pre-labelled during the eclipse phase at 42 °C, including core protein VII. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-30-2-233