@article{mbs:/content/journal/jgv/10.1099/0022-1317-30-2-233, author = "Warocquier, Rita and Boulanger, P. A.", title = "Adenovirus Protein Maturation at 42 °C", journal= "Journal of General Virology", year = "1976", volume = "30", number = "2", pages = "233-242", doi = "https://doi.org/10.1099/0022-1317-30-2-233", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-30-2-233", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Incubation of adenovirus type 2 infected cells at 42 °C resulted in an inhibition of assembly of virus particles although all the major viral structural polypeptides and virus-induced cellular polypeptides so far identified were detected by electrophoretic analysis. Selective high salt-acid-urea extraction of low mol. wt. polypeptides revealed the absence of protein VII at 42 °C whereas precursor polypeptide P-VII and core protein V were found. Pulse-chase and temperature shift experiments indicated that cleavage of P-VII into VII was a reversible thermosensitive process, requiring de novo protein synthesis after shift-down to 37 °C. Virus particles assembled at 37 °C after transfer from 42 to 37 °C contained both viral DNA and polypeptides pre-labelled during the eclipse phase at 42 °C, including core protein VII.", }