1887

Abstract

SUMMARY

Incubation of adenovirus type 2 infected cells at 42 °C resulted in an inhibition of assembly of virus particles although all the major viral structural polypeptides and virus-induced cellular polypeptides so far identified were detected by electrophoretic analysis. Selective high salt-acid-urea extraction of low mol. wt. polypeptides revealed the absence of protein VII at 42 °C whereas precursor polypeptide P-VII and core protein V were found. Pulse-chase and temperature shift experiments indicated that cleavage of P-VII into VII was a reversible thermosensitive process, requiring protein synthesis after shift-down to 37 °C. Virus particles assembled at 37 °C after transfer from 42 to 37 °C contained both viral DNA and polypeptides pre-labelled during the eclipse phase at 42 °C, including core protein VII.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-2-233
1976-02-01
2023-02-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/2/JV0300020233.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-2-233&mimeType=html&fmt=ahah

References

  1. Anderson C. W., Baum P. R., Gesteland R. F. 1973; Processing of adenovirus 2 induced proteins. Journal of Virology 12:241–252
    [Google Scholar]
  2. Boulanger P. A., Puvion F. 1974; Adenovirus assembly. Cross-linking of adenovirus type 2 hexons in vitro. European Journal of Biochemistry 43:465–470
    [Google Scholar]
  3. Eron L., Wesphal H., Callahan R. 1974; In vitro synthesis of adenovirus core proteins. Journal of Virology 14:375–383
    [Google Scholar]
  4. Everitt E., Sundquist B., Pettersson U., Philipson L. 1973; Structural proteins of adenoviruses. X. Isolation and topography of low molecular weight antigens from virion of adenovirus type 2. Virology 52:130–147
    [Google Scholar]
  5. Green M., Pina M. 1963; Biochemical studies on adenovirus multiplication. IV. Isolation, purification and chemical analysis of adenoviruses. Virology 20:199–208
    [Google Scholar]
  6. Ishibashi M., Maizel J. V. 1974; The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology 57:409–424
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage. T4. Nature, London 227:680–685
    [Google Scholar]
  8. Leibowitz J., Horwitz M. S. 1974; Synthesis and assembly of adenovirus type 2 polypeptides. II.Reversible inhibition of hexon assembly at 42°. Virology 61:129–140
    [Google Scholar]
  9. Okubo C. K., Raskas H. J. 1971; Thermosensitive events in the replication of adenovirus type 2 at 42 °C. Virology 46:175–182
    [Google Scholar]
  10. Raska K., Prage J. L., Schlesinger R. W. 1972; Effects of arginine starvation on macromulecular synthesis in infection with type 2 adenovirus. II. Synthesis of virus specific RNA and DNA. Virology 48:472–484
    [Google Scholar]
  11. Rosenwirth B., Tjia S., Westphal M., Doerfler W. 1974; Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2. Virology 60:431–437
    [Google Scholar]
  12. Rouse H. C., Schlesinger R. W. 1967; An arginine-dependent step in the maturation of type 2 adenovirus. Virology 33:513–522
    [Google Scholar]
  13. Rouse H. C., Schlesinger R. W. 1972; The effects of arginine starvation on macromolecular synthesis in infection with type 2 adenovirus. II. Synthesis and utilization of structural proteins. Virology 48:463–471
    [Google Scholar]
  14. Russell W. C., Becker Y. 1968; A maturation factor for adenovirus. Virology 35:18–27
    [Google Scholar]
  15. Russell W. C., Skehel I. J., Williams J. E. 1974; Characterization of temperature-sensitive mutants of adenovirus type 5: synthesis of polypeptides in infected cells. Journal of General Virology 24:247–259
    [Google Scholar]
  16. Samaille J., Warocquier R. 1966; Influence des temperatures supra-optimales sur le cycle de multiplication de l’adenovirus 5. Annales de VInstitut Pasteur de Paris no:244–251
    [Google Scholar]
  17. Sundquist B., Everiitt E., Philipson L., Hoglund S. 1973; Assembly of adenoviruses. Journal of Virology n:449–459
    [Google Scholar]
  18. Warocquier R., Boulanger P. A. 1972; Adenovirus metabolism at non permissive temperature.1.Activity of viral polysomes. Archiv fur die Gesamte Virusforschung 37:275–278
    [Google Scholar]
  19. Warocquier R., Boulanger P. A. 1973; Adenovirus protein metabolism at non permissive temperature. Transfer of viral polypeptides to nucleus and evidence for a thermostable infected cell specific polypeptides. Archiv fur die Gesamte Virusforschung 41:371–381
    [Google Scholar]
  20. Warocquier R., Menard D., Samaille J. 1966; Technique rapide de titrage des particules infectieuses de l’adenovirus 5. Annales de VInstitut Pasteur de Lille 17:97–106
    [Google Scholar]
  21. Warocquier R., Samaille J. 1968; Action de 1’actinomycine D, de la puromycine et de la p-fluoro-phenylalanine sur la phase thermosensible du cycle de multiplication de l’adenovirus 5. Comptes Rendu de VAcademie des Sciences de Paris 266:2513–2515
    [Google Scholar]
  22. Warocquier R., Samaille J., Green M. 1969; Biochemical studies on adenovirus multiplication. XVI. Transcription of the adenovirus genome during abortive infection at elevated temperatures. Journal of Virology 4:423–428
    [Google Scholar]
  23. White A. D., Scharff M. D., Maizel J. V. 1969; The polypeptides of adenovirus. III. Synthesis in infected cells. Virology 39:395–406
    [Google Scholar]
  24. Winters W. D., Russell W. C. 1971; Studies on the assembly of adenovirus in vitro. Journal of General Virology 10:181–184
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-2-233
Loading
/content/journal/jgv/10.1099/0022-1317-30-2-233
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error