1887

Abstract

SUMMARY

Controlled disruption of 60S AMV RNA with formamide was used to prepare 50–55S and 30–40S RNAs. When the activities of these RNAs as templates for AMV reverse transcriptase were compared it was found that 50–55S RNA was 1.5 times and 30–40S RNA 2 to 3 times more active than 60S RNA. The 30–40S RNA produced by heating, instead of formamide disruption, was inactive as a template but activity was restored by addition of oligo(dT). 40% of the 4S RNA initially associated with the 60S RNA remained associated with all the RNA species obtained by formamide treatment but was lost on heating. It is concluded that this RNA acts as resident primer whereas the other 60% of the 4S RNA is less firmly bound and appears to have little or no primer activity. Removal of the less firmly bound 4S RNA increases the template activity of the viral RNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-2-187
1976-02-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/2/JV0300020187.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-2-187&mimeType=html&fmt=ahah

References

  1. Baltimore D., Smoler D. 1971; Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 68:1507–1511
    [Google Scholar]
  2. Canaani E., Duesberg P. H. 1972; Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase. Journal of Virology 10:23–31
    [Google Scholar]
  3. Dahlberg J. E., Sawyer R. C., Taylor J. M., Faras A. J., Levinson W. E., Goodman H. M., Bishop J. M. 1974; Transcription of DNA from the 70S RNA of Rous sarcoma virus. I. Identification of a specific 4S RNA which serves as primer. Journal of Virology 13:1126–1133
    [Google Scholar]
  4. Duesberg P. H. 1968; Physical properties of Rous sarcoma virus RNA. Proceedings of the National Academy of Sciences of the United States of America 60:1511–1518
    [Google Scholar]
  5. Duesberg P. H., Helm, Von Der K., Canaani E. 1971a; Properties of a soluble DNA polymerase isolated from Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 68:747–751
    [Google Scholar]
  6. Duesberg P. H., Helm, Von Der K., Canaani E. 1971b; Comparative properties of RNA and DNA templates for the DNA polymerase of Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 68:2505–2509
    [Google Scholar]
  7. Erikson R. L. 1969; Studies On the RNA from avian myeloblastosis vims. Virology 37:124–131
    [Google Scholar]
  8. Erikson E., Erikson R. L. 1971; Association of 4S ribonucleic acid with oncornavirus ribonucleic acids. Journal of Virology 8:254–256
    [Google Scholar]
  9. Faras A. J., Taylor J. M., Mcdonell J. P., Levinson W. E., Bishop J. M. 1972; Purification and characterization of deoxyribonucleic acid polymerase associated with Rous sarcoma virus. Biochemistry n:2334–2342
    [Google Scholar]
  10. Faras A. J., Garapin A. C., Bishop J. M., Goodman H. M. 1973; Characterization of the low-molecular-weight RNAs associated with the 70S RNA of Rous sarcoma virus. Journal of Virology 12:334–342
    [Google Scholar]
  11. Faras A. J., Dahlberg J. E., Sawyer R. C., Harada F., Taylor J. M., Levinson W. E., Bishop J. M., Goodman H. M. 1974; Transcription of DNA from the 70S RNA of Rous sarcoma virus. Journal of Virology 13:1134–1142
    [Google Scholar]
  12. Goodman N. C., Spiegelman S. 1971; Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proceedings of the National Academy of Sciences of the United States of America 68:2203–2206
    [Google Scholar]
  13. Kacian D. L., Watson K. F., Burny A., Spiegelman S. 1971; Purification of the DNA polymerase of avian myeloblastosis virus. Biochimica et Biophysica Acta 246:365–383
    [Google Scholar]
  14. Kisselev L. L., Frolova L. YU., Gazaryan K. G., Tarantul V. Z., Engelgardt V. A. 1973; The synthesis of DNA complementary to pigeon globine mRNA using reverse transcriptase. (In Russian.) Proceedings of the Academy of Sciences of the USSR 213:1203–1206
    [Google Scholar]
  15. Leis J. P., Hurwitz J. 1972; RNA-dependent DNA polymerase activity of RNA tumor virus. II. Directing influence of RNA in the reaction. Journal of Virology 9:130–142
    [Google Scholar]
  16. Mangel W. F., Delius H., Duesberg P. H. 1974; Structure and molecular weight of the 60-70S RNA and the 30-40S RNA of the Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 71:4541–4545
    [Google Scholar]
  17. Maugh T. H. 1974; Rous sarcoma virus: a new role for transfer RNA. Nature, London 251:41
    [Google Scholar]
  18. Reitz M., Gillespie D., Saxinger W. C., Robert M., Gallo R. C. 1972; Poly(lA) tracts Of tumor virus 70S RNA are not transcribed in endogenous or reconstituted reactions of viral reverse transcriptase. Biochemical and Biophysical Research Communications 49:1216–1224
    [Google Scholar]
  19. Rosenthal L. J., Zamecnik P. C. 1973; Minor base composition of‘70S-associated’ 4S RNA from avian myeloblastosis virus. Proceedings of the National Academy of Sciences of the United States of America 70:865–869
    [Google Scholar]
  20. Říman J., Beaudreau G. S. 1970; Viral DNA-dependent DNA polymerase and the properties of thymidine labelled material in virions of an oncogenic RNA virus. Nature, London 228:427–430
    [Google Scholar]
  21. Sawyer R. C., Harada F., Dahlberg J. E. 1974; Virion-associated RNA primer for Rous sarcoma virus DNA synthesis: isolation from uninfected cells. Journal of Virology 13:1302–1311
    [Google Scholar]
  22. Spiegelman S., Burny A., Das M. R., Schlom J., Trávníček M., Watson K. 1970; Characterization of the products of RNA-directed DNA polymerase in oncogenic RNA viruses. Nature, London 227:563–567
    [Google Scholar]
  23. Tiollais P., Galibert F., Lepetit A., Auger M.A. 1972; L’electrophorese des acides ribonucleiques en gel de polyacrylamide. Biochimie 54:339–354
    [Google Scholar]
  24. Trávníček M., Říman J. 1973a; Occurrence of aminoacyl-tRNA synthetase in an RNA oncogenic virus. Nature New Biology 241:60–62
    [Google Scholar]
  25. Trávníček M., Říman J. 1973b; Subunits of oncornavirus high-molecular-weight RNA. I. Stepwise conversion of 60S AMV (avian myeloblastosis virus) RNA to subunits. Biochemical and Biophysical Research Communications 53:217–223
    [Google Scholar]
  26. Trávníček M., Říman J. 1973c; Subunits of on cornavirus high-molecular-weight RNA. II. Detection of double-stranded-regions in 60S AMV (avian myeloblastosis virus) RNA. Biochemical and Biophysical Research Communications 54:1347–1355
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-2-187
Loading
/content/journal/jgv/10.1099/0022-1317-30-2-187
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error