1887

Abstract

SUMMARY

Four temperature sensitive mutants of adenovirus type 12, classified into complementation group D, synthesize most or all of the late virus-specific polypeptides at the restrictive temperature, as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Infections by these mutants under restrictive conditions apparently result in some over production of the virion hexon polypeptide compared to wild type infection. Genetic recombination between the four mutants has been demonstrated. A partial linear genetic map of the D cistron is presented.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-1-153
1976-01-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/1/JV0300010153.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-1-153&mimeType=html&fmt=ahah

References

  1. Ensinger M. J., Ginsberg H. S. 1972; Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus. Journal of Virology 10:329–339
    [Google Scholar]
  2. Ginsberg H. S., Ensinger M. J., Kauffman R. S., Mayer A. J., Lundholm U. 1974; Cell transformation: a study of regulation with types 5 and 12 adenovirus temperature-sensitive mutants. Cold Spring Harbor Symposia on Quantitative Biology 39:419–426
    [Google Scholar]
  3. Ledinko N. 1967; Stimulation of DNA synthesis and thymidine kinase activity in human embryonic kidney cells infected by adenovirus 2 or 12. Cancer Research 27:1459–1469
    [Google Scholar]
  4. Ledinko N. 1972; Nucleolar ribosomal precursor RNA and protein metabolism in human embryo kidney cultures infected with adenovirus 12. Virology 49:79–89
    [Google Scholar]
  5. Ledinko N. 1973; Nuclear acidic protein changes in adenovirus-infected human embryo kidney cultures. Virology 54:294–298
    [Google Scholar]
  6. Ledinko N. 1974; Temperature-sensitive mutants of adenovirus type 12 defective in viral DNA synthesis. Journal of Virology 14:457–468
    [Google Scholar]
  7. Maizel J. V. Jun, White D. O., Scharff M. D. 1968; The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7a and 12. Virology 36:115–125
    [Google Scholar]
  8. Russell W. C., Skehel J. J. 1973; The polypeptides of adenovirus type 12. Journal of General Virology 20:195–209
    [Google Scholar]
  9. Shimojo H., Shiroki K., Yamaguchi K. 1974; The viral dna replication machinery of adenovirus 12. Cold Spring Harbor Symposia on Quantitative Biology 39:533–538
    [Google Scholar]
  10. Shiroki K., Irisawa J., Shimojo H. 1972; Isolation and a preliminary characterization of temperature-sensitive mutants of adenivorus 12. Virology 49:1
    [Google Scholar]
  11. Tibbetts C., Johansson K., Philipson L. 1973; Hydroxyapatite chromatography and formamide de-naturation of adenovirus deoxyribonucleic acid. Journal of Virology 12:218–225
    [Google Scholar]
  12. Williams J. F., Young C. S. H., Austin P. E. 1974; Genetic analysis of human adenovirus type 5 in permissive and non-permissive cells. Cold Spring Harbor Symposia on Quantitative Biology 39:427–437
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-1-153
Loading
/content/journal/jgv/10.1099/0022-1317-30-1-153
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error