1887

Abstract

SUMMARY

Twenty African arboviruses, five alphaviruses, nine flaviviruses, three Bunyamwera Group viruses, two Bwamba Group viruses and one ungrouped virus were titrated in parallel in 11 cell systems, in suckling mice and adult mice. The relative sensitivities of the and systems have been compared.

The highest infectivities were obtained in suckling mice. Vero and LLC-MK2 cells produced plaques with the greatest number of viruses and Semliki Forest virus grew most readily. Ntaya virus and Dengue 1 virus were difficult to culture and Zika virus yielded better in cell culture than in adult or suckling mice.

and neutralization tests were made on human sera in groups of 50. Each group of sera was tested against one of five viruses, representatives of three of the arbovirus groups titrated. Good agreement was obtained between the two test systems with West Nile, O’nyong-nyong and Wesselsbron viruses but there were significant differences in results obtained with Germiston and Pongola viruses.

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1976-01-01
2024-04-24
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References

  1. Bowen E. T. W., Simpson D. I. H., Platt G. S., Way H., Bright W. F., Day J., Achapa S., Roberts J. M. D. 1973; Large scale irrigation and arbovirus epidemiology, Kano Plain, Kenya. II. Preliminary serological survey. Transactions of the Royal Society of Tropical Medicine and Hygiene 67:702–709
    [Google Scholar]
  2. Bradish C. J., Allner K. 1966; The assay of Langat and louping ill viruses and their specific antisera in agar suspensions of chick embryo fibroblasts. Proceedings of the Conference on Rapid Identification of Biological Agents Washington, D.C.: Office of Naval Research;
    [Google Scholar]
  3. Buckley S. M. 1964; Applicability of the HeLa(Gey) strain of human malignant epithelial cells to the propagation of arboviruses. Proceedings of the Society for Experimental Biology and Medicine 116:354–358
    [Google Scholar]
  4. Cooper P. D. 1955; A method for producing plaques in agar suspensions of animal cells. Virology 1:397–401
    [Google Scholar]
  5. David-West T. S. 1972; Tissue culture studies of common Nigerian arboviruses. Propagation in different tissue culture systems. West African Medical Journal 21:3–9
    [Google Scholar]
  6. Dulbecco R. 1952; Production of plaques in monolayer tissue cultures by single particles of an animal virus. Proceedings of the National Academy of Sciences of the United States of America 38:747–752
    [Google Scholar]
  7. Fisher R. A. 1946 Statistical Methods for Research Workers 10th edition, chapter iv, pp. 96–113 Edinburgh: Oliver & Boyd Ltd;
    [Google Scholar]
  8. Henderson J. R., Taylor R. M. 1960; Propagation of certain arboviruses in avian and primate cell cultures. The Journal of Immunology 84:590–598
    [Google Scholar]
  9. Karabatsos N., Buckley S. M. 1967; Susceptibility of the baby-hamster kidney-cell line (BHK 21) to infection with arboviruses. The American Journal of Tropical Medicine and Hygiene 16:99–105
    [Google Scholar]
  10. Porterfield J. S. 1960; A simple plaque-inhibition test for the study of arboviruses. Bulletin of The World Health Organization 22:373–380
    [Google Scholar]
  11. Smith C. E. G., Westgarth D. R. 1957; The use of survival time in the analysis of neutralization tests for serum antibody. The Journal of Hygiene, Cambridge 55:224–238
    [Google Scholar]
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