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A virus from adult kelp flies (Chaetocoelopa sydneyensis) has been cultured in larvae of the waxmoth (Galleria mellonella). This virus has isometric particles that are 29 ± 1 nm in diameter and resemble reovirus particles in appearance. Some chemical and physical properties of these particles have been determined. They contain single-stranded RNA, but have a unique set of the properties of the particles reported for other picornaviruses, and differ from all in surviving for 10 min at 90 °C but not 100 °C. The cryptogram of kelp fly virus is R/1:3.5/*:S/S:I/*.