1887

Abstract

SUMMARY

Virus-free buffer extracts of vaccinia-infected sheep dermis were fractionated by elution from DEAE-cellulose with increasing concentrations of NaCl. The fraction eluted with 4.0-NaCl was serologically homogeneous when examined in precipitation-in-gel tests. Further fractionation on Sephadex G-200 gave a material which was also physically homogeneous when examined by both ultracentrifugal and electrophoretic techniques. A sedimentation coefficient of 3.47 and a molecular weight of 31,000 were calculated from ultracentrifugal data. The isolated material contained 74.3% DNA, 9.4% protein estimated as bovine serum albumin and 14.9% carbohydrate estimated as glucose. The DNA contained the bases adenine, thymine, guanine and cytosine in the ratios 1.0:1.1:0.8:0.8. Paper chromatography of formic-acid hydrolysates of the material resolved seven substances reacting with ninhydrin. Qualitative colorimetric tests indicated, apart from deoxyribose, the presence of a hexose and a hexuronic acid.

The serological activity of the isolated material was heat-stable and identical with that of a previously described heat-stable extract of vaccinia-infected rabbit dermis. Treatment with enzymes indicated the presence of two different, serologically active sites. Failure to elicit an antibody response following injection into rabbits suggested that the isolated material was a hapten. Serum absorption studies showed serological identity with an antigen present on the surface of the vaccinia virus particle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-3-3-359
1968-12-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/3/3/JV0030030359.html?itemId=/content/journal/jgv/10.1099/0022-1317-3-3-359&mimeType=html&fmt=ahah

References

  1. Appleyard G. 1961; An immunizing antigen from rabbit-pox and vaccinia virus. Nature, Land 190:465
    [Google Scholar]
  2. Appleyard G., Zwartouw H. T., Westwood J. C. N. 1964; Aprotective antigen from poxviruses. Br. J. exp. Path 45:150
    [Google Scholar]
  3. Archibald W. J. 1947; A demonstration of some methods of determining molecular weights from the data of the ultracentrifuge. J. phys. Colloid Chem 51:1204
    [Google Scholar]
  4. Chargaff E. 1955 In The Nucleic Acids vol 1 p 352 Ed. by Chargaff E., Davidson J. N. New York: Academic Press, Inc;
    [Google Scholar]
  5. Ch’en W. K. 1934; Preparation of specific soluble substance from vaccinia virus. Proc. Soc. exp. Biol. Med 32:491
    [Google Scholar]
  6. Cohen G. H., Wilcox W. C. 1966; Soluble antigens of vaccinia-infected mammalian cells 1. Separation of virus-induced protein into two classes on the basis of physical characteristics. J. Bact. 92:676
    [Google Scholar]
  7. Craigie J. 1932; The observation of the vaccinia flocculation reaction, and observations of the elementary bodies of vaccinia. Br. J. exp. Path. 13:259
    [Google Scholar]
  8. Craigie J., Wishart F. O. 1936; Studies on soluble precipitable substances of vaccinia: soluble precipitable substances of dermal filtrate. J. exp. Med. 64:819
    [Google Scholar]
  9. Dische Z. 1930; Ueber einige neue charakteristische Farbreaktionen der Thymonukleinsaeure und eine Mikromethode zur Bestimmung derselben in tierischen Organen mit Hilfe dieser Reaktionen. Mikrochemie 8:4
    [Google Scholar]
  10. Dische Z. 1947; A new colour reaction of hexuronic acids. J. biol. Chem 167:189
    [Google Scholar]
  11. Dische Z. 1949; Spectrophotometric method for the determination of free pentose and pentose in nucleotides. J. biol. Chem 181:379
    [Google Scholar]
  12. Dische Z., Shettles L. B., Osnos M. 1949; New specific colour reactions of hexoses and spectrophotometric micromethods for their determination. Archs. Biochem 22:169
    [Google Scholar]
  13. Easterbrook K. B. 1966; Controlled degradation of vaccinia virus in vitro: an electron microscopic study. J. Ultrastruct. Res. 14:484
    [Google Scholar]
  14. Flodin P. 1962; Dextran gels and their applications in gel filtration. Ph.D. Thesis Uppsala University;
    [Google Scholar]
  15. Gispen R. 1955; Analysis of poxvirus antigens by means of double-diffusion. A method for the direct serological differentiation of cowpox. J. Jmmun 74:134
    [Google Scholar]
  16. Hayward B. J., Augustin R. 1957; Quantitative gel-diffusion methods for assay of antigens and antibodies. Int. Archs Allergy appl. Immun 11:192
    [Google Scholar]
  17. Joklik W. K. 1962a; Purification of four strains of poxvirus. Virology 18:9
    [Google Scholar]
  18. Joklik W. K. 1962b; The preparations and characteristics of highly purified radioactively labelled poxvirus. Biochim. biophys. Acta 61:290
    [Google Scholar]
  19. Levin C. 1958; Chromatography of tobacco mosaic virus and potato virus K. Archs Biochem. Biophys 78:33
    [Google Scholar]
  20. Marquardt J., Holm S. E., Lycke E. 1965; Immunoprecipitating factors of vaccinia virus. Virology 27:170
    [Google Scholar]
  21. Morris D. L. 1948; Qualitative determination of carbohydrates with Dreywood’s anthrone reagent. Science, N. Y 107:254
    [Google Scholar]
  22. Ornstein L., Davis B. J. 1962; Disc electrophoresis. Preprinted by Distillation Products Industries, Eastman Kodak Co;
    [Google Scholar]
  23. Parker R. F., Rivers T. M. 1935; Immunological and chemical investigations of vaccine virus. 1. Preparation of elementary bodies of vaccinia. J. exp. Med 62:65
    [Google Scholar]
  24. Parker R. F., Rivers T. M. 1937; Immunological and chemical investigations of vaccine virus. VI. Isolation of a heat-stable, serologically active substance from tissues infected with vaccine virus. J. exp. Med 65:243
    [Google Scholar]
  25. Pfau C. J., McCrea J. F. 1962; Release of deoxyribonucleic acid from vaccinia virus with 2-mercaptoethanol and pronase. Nature, Lond 194:894
    [Google Scholar]
  26. Rodriguez-Burgos A., Chordi A., Diaz R., Tormo J. 1966; Immunoelectrophoretic analysis of vaccinia virus. Virology 30:569
    [Google Scholar]
  27. Rondle C. J. M., Dumbell K. R. 1962; Antigens of cowpox virus. J. Hyg., Camb 60:41
    [Google Scholar]
  28. Rondle C. J. M., Williamson J. D. 1968; Studies on the LS antigen of vaccinia virus. J. Hyg., Camb 66:507
    [Google Scholar]
  29. Smadel J. E., Lavin G. I., Dubos R. I. 1940; Some constituents of elementary bodies of vaccinia. J. exp. Med 71:373
    [Google Scholar]
  30. Smadel J. E., Rivers T. M. 1942; The LS antigen of vaccinia. 1. Inhibition of L- and S-antibodies by substances in treated vaccine dermal filtrate. J. exp. Med 75:151
    [Google Scholar]
  31. Smith W. 1932; A heat-stable precipitating substance extracted from vaccinia virus. Br. J. exp. Path 13:434
    [Google Scholar]
  32. Stumpf P. J. 1947; A colorimetric method for the determination of dexoyribonucleic acid. J. biol. Chem 169:367
    [Google Scholar]
  33. Sutherland E. W., Cori C. F., Haynes R., Olsen A. S. 1949; Purification of the hyperglycemic-glycogenolytic factor from insulin and from gastric mucosa. J. biol. Chem 180:825
    [Google Scholar]
  34. Westwood J. C. N., Zwartouw H. T., Appleyard G., Titmuss D. H. J. 1965; Comparison of the soluble antigens and virus particle antigens of vaccinia virus. J. gen. Microbiol 38:47
    [Google Scholar]
  35. Williamson J. D. 1963; Vaccinia soluble antigen. Ph.D. Thesis Liverpool University;
    [Google Scholar]
  36. Williamson J. D., Rondle C. J. M. 1964; A virus-specific hapten from vaccinia-infected sheep dermis. Biochem. J 90:13 P
    [Google Scholar]
  37. Wyatt G. R., Cohen S. S. 1953; The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine. Biochem. J 55:774
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-3-3-359
Loading
/content/journal/jgv/10.1099/0022-1317-3-3-359
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error