Sarcomas induced in 13 BALB/c mice, one Sprague-Dawley rat and five Syrian (golden) hamsters by the murine sarcoma virus-harvey (MSV-h) were grown in cell culture and the cell lines maintained for a maximum of 194 days. The majority of the tumour lines formed three-dimensional colonies of densely packed cells which eventually broke free from the glass and floated in the medium. Infective MSV-h was obtained from the mouse and rat tumour cultures. Filtrates of the culture fluids induced typical MSV-h lesions when injected into newborn mice, and those from cultures maintained for 4 to 6 months were as effective as those from cultures maintained for shorter periods. The newborn mice receiving undiluted culture fluids rapidly developed sarcomas and erythroblastic splenomegaly, while recipients of limiting dilutions developed lymphocytic leukaemia which never appeared earlier than 2 months after injection. Thus, the MSV-h derived from cell cultures behaved in the same way as that derived from infected animals.
However, hamster tumour-cell culture filtrates did not contain active MSV-h when tested in mice, but one such filtrate induced typical sarcomas and other characteristic MSV-h lesions when injected into newborn hamsters.
BassinR. H.
,
SimonsP. J.
,
ChestermanF. C.
,
HarveyJ. J.
.
1968; Murine Sarcoma Virus (HARVEY). Characteristics of focus formation in mouse embryo cell cultures and virus production by hamster tumor cells. Int. J. Cancer 3:265
BernardC.
,
BoironN. M.
,
LasneretJ.
.
1967; Transformation et infection chronique de cellules embryonnaires de rat par le virus du sarcome de Moloney. C. r. hebd. Séanc. Acad. Sci., Paris 264:2170
ChestermanF. C.
,
HarveyJ. J.
,
DourmashkinR. R.
,
SalamanM. H.
.
1966; The pathology of tumours and other lesions induced in rodents by virus derived from a rat with Moloney leukaemia. Cancer Res 26:1759
HartleyJ. W.
,
RoweW. P.
.
1966; Production of altered cell foci in tissue culture by defective Moloney Sarcoma Virus. Proc. natn. Acad. Sci.U. S.A 55:780
HuebnerR. J.
,
HartleyJ. W.
,
RoweW. P.
,
LaneW. T.
,
CappsW. J.
.
1966; Rescue of the defective genome of Moloney Sarcoma Virus from a noninfectious hamster tumour and the production of pseudotype sarcoma viruses with various murine leukaemia viruses. Proc. natn. Acad. Sci.U. S.A 56:1164
MoloneyJ. B.
.
1960; Biological studies on a lymphoid-leukaemia virus extracted from Sarcoma 37,. I. Origin and introductory investigations. J. natn. Cancer Inst 24:933
MoloneyJ. B.
.
1966a In Some Recent Developments in Comparative Medicine Ed. by
-FiennesR. N. T.-W.
.
Symposia of the Zoological Society of London, no. 17, p 251 London: Academic Press;
RauscherJ. R.
,
ReyniersJ. A.
,
SackstederM. R.
.
1964; Response or lack of response of apparently leukosis-free Japanese quail to avian tumour viruses. Natn. Cancer Inst. Monograph No. 17 211
SanfordK. K.
,
BarkerB. E.
,
WoodsM. W.
,
ParshadR.
,
LawL. W.
.
1967; Search for indicators of neoplastic conversion ‘in vitro’
. J. natn. Cancer Inst 39:705
SimonsP. J.
,
BassinR. H.
,
HarveyJ. J.
.
1967; Transformation of hamster embryo cells in vitro by Murine Sarcoma Virus (HARVEY). Proc. Soc. exp. Biol. Med 125:1242
SimonsP. J.
,
DourmashkinR. R.
,
TuranoA.
,
PhillipsD. E. H.
,
ChestermanF. C.
.
1967; Morphological transformation of mouse embryo cells in vitro by Murine Sarcoma Virus (HARVEY). Nature, Land 214:897