Sarcomas induced in 13 BALB/c mice, one Sprague-Dawley rat and five Syrian (golden) hamsters by the murine sarcoma virus- (MSV-) were grown in cell culture and the cell lines maintained for a maximum of 194 days. The majority of the tumour lines formed three-dimensional colonies of densely packed cells which eventually broke free from the glass and floated in the medium. Infective MSV- was obtained from the mouse and rat tumour cultures. Filtrates of the culture fluids induced typical MSV- lesions when injected into newborn mice, and those from cultures maintained for 4 to 6 months were as effective as those from cultures maintained for shorter periods. The newborn mice receiving undiluted culture fluids rapidly developed sarcomas and erythroblastic splenomegaly, while recipients of limiting dilutions developed lymphocytic leukaemia which never appeared earlier than 2 months after injection. Thus, the MSV- derived from cell cultures behaved in the same way as that derived from infected animals.

However, hamster tumour-cell culture filtrates did not contain active MSV- when tested in mice, but one such filtrate induced typical sarcomas and other characteristic MSV- lesions when injected into newborn hamsters.


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