%0 Journal Article %A Mayo, M. A. %A Cocking, E. C. %T Double Staining of Tobacco-mosaic-virus-infected Material Using Phosphotungstic Acid and Uranyl Acetate During Dehydration %D 1968 %J Journal of General Virology, %V 3 %N 2 %P 309-310 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-3-2-309 %I Microbiology Society, %X The most widely used staining procedure in recent electron microscope studies of virus-infected plants has involved the use of uranyl acetate, either during dehydration or as a post-stain, together with lead citrate as a post-stain (Arnott & Smith, 1968; Milne & de Zoeten, 1967). Following the recent demonstration that the appearance of tobacco mosaic virus in thin section could be altered by prolonging the duration of dehydration staining with uranyl acetate (Cocking & Pojnar, 1968a), the use of other heavy-metal stains for dehydration staining, both alone and in combination with uranyl acetate, has been investigated. A double-staining procedure using phosphotungstic acid and uranyl acetate has been developed and the potential of this procedure in electron-microscope studies of virus infection is discussed. Phosphotungstic acid has been known for many years to give high contrast when used as a tissue stain during dehydration (Huxley, 1958). %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-3-2-309