Electron Microscopic Observations of Colorado Tick Fever Virus in BHK 21 and KB Cells Free

Abstract

Electron-microscopic studies have revealed intracytoplasmic virus particles and unusual intracytoplasmic and intranuclear fibrillar structures in BHK 21 and KB cells infected with Colorado tick fever (CTF) virus. This communication briefly describes these findings.

BHK 21 cells were grown in Eagle’s basal medium (BME) in Hanks’s balanced salt solution (BSS) + 10% foetal bovine serum. Fortified BME in Earle’s BSS containing 10% foetal bovine serum was used as the outgrowth medium for KB cells. The monolayers were grown in 8 oz prescription bottles and were infected with 0.5 ml. of the florio strain of CTF virus (10 TCD 50/ml.) in 10 ml. of maintenance medium. The maintenance medium for BHK 21 cells consisted of BME in Earle’s BSS and 3% foetal bovine serum. Leibovitz 15 (L-15) medium (Leibovitz, 1963) containing 2% inactivated foetal bovine serum was used as the maintenance medium for KB cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-3-2-279
1968-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/3/2/JV0030020279.html?itemId=/content/journal/jgv/10.1099/0022-1317-3-2-279&mimeType=html&fmt=ahah

References

  1. Leibovitz A. 1963; the growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. hyg 78:173
    [Google Scholar]
  2. Rifkind R. A., Hsu K. C., Morgan C. 1964; immunochemical staining for electron microscopy. J. histochem. cytochem 12:131
    [Google Scholar]
  3. Singer S. J. 1959; preparation of an electron-dense antibody conjugate. nature, lond 183:1523
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-3-2-279
Loading
/content/journal/jgv/10.1099/0022-1317-3-2-279
Loading

Data & Media loading...

Most cited Most Cited RSS feed